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芹菜在衰老过程中,叶绿素不断降解,过氧化物酶活性升高而SOD活性逐渐降低,与此同时,膜脂质过氧化作用逐渐增强,表现为脂质过氧化产物──丙二醛不断积累,导致细胞膜透性逐渐增大。外源赤霉素(GA)处理可以明显抑制芹菜衰老过程中的脂质过氧化作用,延缓芹菜的衰老。,达极显著水平。图2各处理PO0、SOD活性的变化(叶柄)在:SOD活性是以初始值为100的相对活性。2.4GA处理芹菜丙二醛(MDA)含量的变化图3各处理叶柄MDA含量的变化由图3可以看出,作为膜脂过氧化产物之一──丙二醛,在芹菜衰老过程中呈累积性上升,GA处理,可以极显著地(α=0.0l)抑制MDA积累,2.5芹菜衰老过程中细胞膜透性的变化表2GA处理叶柄细胞膜透性的变化相对电导率%由表2的结果可以看出,随着实验期的延长,芹菜的细胞膜透性逐渐增大,GA处理,可显著地降低膜透性的变化,保护膜结构的完整性。3讨论与结论目前,关于植物衰老的研究表明,膜脂过氧化对植物的衰老具有促进与加强作用[4]。本试验的结果表明,芹菜衰老过程中,在叶绿素降解的同时,SOD活性下降,脂质过氧化产物──MDA积累,导致细胞内膜系统的连锁性破坏。并且越到后期这种破坏作用越为明显,?
Celery in the aging process, chlorophyll continued degradation, peroxidase activity increased while SOD activity decreased, at the same time, the membrane lipid peroxidation gradually increased, the performance of lipid peroxidation products ─ ─ continue to accumulate malondialdehyde , Leading to the gradual increase of cell membrane permeability. Exogenous gibberellin (GA) treatment can significantly inhibit the lipid peroxidation of celery aging process, delay the aging of celery. , Reaching a significant level. Fig.2 Changes of PO0 and SOD activities (petioles) in each treatment: The SOD activity is based on the relative activity of the initial value of 100. 2.4GA treatment of celery malondialdehyde (MDA) content changes in Figure 3 treatment leaf petiole MDA content changes can be seen from Figure 3, as one of the membrane lipid peroxidation products ─ ─ malondialdehyde, celery aging was Cumulative increase, GA treatment, can significantly (α = 0.0l) inhibition of MDA accumulation, 2.5 celery senescence process of cell membrane permeability changes Table 2GA treatment petiole membrane permeability changes relative conductivity% from Table 2 The results can be seen, with the extension of the experimental period, celery membrane permeability gradually increased, GA treatment, can significantly reduce the membrane permeability changes to protect the integrity of the membrane structure. DISCUSSION AND CONCLUSIONS At present, studies on plant senescence show that membrane lipid peroxidation can promote and enhance plant senescence [4]. The results of this experiment showed that during the process of celery aging, while the chlorophyll was degraded, the SOD activity decreased and the lipid peroxidation product ─ ─ MDA accumulated, leading to the chain destruction of the cell membrane system. And more damage to the more obvious later,?