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为比较不同赤眼蜂酚氧化酶(PO)活性的大小,明确赤眼蜂种间免疫防御能力强弱,本研究通过研磨、离心提取松毛虫赤眼蜂Trichogramma dendrolimi、螟黄赤眼蜂T.chilonis、玉米螟赤眼蜂T.ostriniae匀浆液,首次测定了几种化学因子(Ca2+、昆布多糖、纤维素、右旋糖酐、脂多糖和丝氨酸蛋白酶抑制剂)对3种供试赤眼蜂匀浆液中酚氧化酶原激活系统(proPO-AS)的激活程度。结果表明:玉米螟赤眼蜂匀浆液PO活性最高,螟黄赤眼蜂次之,松毛虫赤眼蜂最低。Ca2+是激活供试赤眼蜂proPO的必需因子,且在0.03mol/L浓度下激活作用最强;昆布多糖和脂多糖也能有效激活proPO,0.01及0.05mg/mL昆布多糖能强烈激活PO活性;纤维素和右旋糖酐对PO活性存在显著抑制作用。结果证明了赤眼蜂种间存在明显的免疫能力差异。本研究建立了小型昆虫proPO-AS研究体系,为研究Wolbachia-小型昆虫的免疫互作体系奠定了基础。
In order to compare the activity of different erythropolis of phenoloxidase (PO) and clarify the immune defense ability between Trichogramma species, Trichogramma dendrolimi and Trichogramma dendrolimi were extracted by grinding and centrifugation. chilonis, T. ostriniae homogenates of Trichogramma ostriniae were tested for the first time in three homozygotes Trichogramma homozygotes of several chemical agents (Ca2 +, laminaran, cellulose, dextran, lipopolysaccharide and serine protease inhibitors) Activation of proPO-AS. The results showed that the activity of PO was the highest in Trichogramma ostriniae homogenate, followed by Trichogramma ostriniae and Trichogramma ostriniae. Ca2 + is an essential factor to activate proPO in experimental Trichogramma and has the strongest activation in the concentration of 0.03mol / L. Laminopolysaccharide and lipopolysaccharide can also activate proPO effectively. 0.01 and 0.05mg / mL laminarin can strongly activate PO activity ; Cellulose and dextran significantly inhibited the activity of PO. The results demonstrate that Trichogramma species have significant differences in immunocompetence. This study established the research system of proPO-AS for small insects, which lays the foundation for the research of immune interaction system of Wolbachia-small insects.