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细菌性败血症对鱼类危害严重,为探究鲂属鱼类不同抗性品种响应病原菌嗜水气单胞菌的抗性机制,以三角鲂(高抗)和团头鲂(易感)肝组织为材料,采用非标记定量蛋白质组学技术分析其在嗜水气单胞菌侵染胁迫后3、10和24h与对照组0h(未感染)肝组织蛋白质组的变化,对差异表达蛋白质进行质谱鉴定、相对定量和生物信息学分析。结果显示:三角鲂和团头鲂在嗜水气单胞菌侵染下,分别有46个和29个蛋白质在侵染后各时期的表达丰度相比对照都发生了显著变化。通过基因本体(gene ontology,GO)功能注释发现,相对于团头鲂,三角鲂中氧化还原蛋白质比例从7%增加到13%,且发现一类新的β-免疫球蛋白(β-globin)在各时期分别上调表达了1.64、3.44和1.93倍。生物信息学分析进一步表明,三角鲂在响应嗜水气单胞菌侵染过程中可能特异性地启动了包括戊糖磷酸途径、脂肪酸代谢、亮氨酸代谢途径和β-globin高表达的协同抗性机制,从而抵御病原菌入侵。该研究成果为后续深入揭示鱼病互作分子机制及鲂属鱼类抗病新品种选育提供了重要的前期理论研究基础。
Bacterial sepsis is very harmful to fish. In order to explore the resistance mechanism of different resistant varieties to the pathogenic bacterium Aeromonas hydrophila, the results showed that the triglyceride (high resistance) and bream (susceptible) liver tissue were Materials, using unlabeled quantitative proteomics analysis of changes in the proteome of the liver tissue 3h, 10h and 24h after infection with Aeromonas hydrophila and control group 0h (uninfected), the differential expression of proteins were identified by mass spectrometry , Relative quantification and bioinformatics analysis. The results showed that the expression abundance of 46 and 29 proteins changed significantly after infection compared with the control under the infection of Aeromonas hydrophila. According to the gene ontology (GO) functional annotation, it was found that the proportion of redox proteins in the triangulus increased from 7% to 13% with respect to the bream, and a new class of β-globin was found In each period, they raised 1.64, 3.44 and 1.93 times respectively. Bioinformatics analysis further demonstrated that Triangulation may specifically initiate a synergistic combination of pentose phosphate pathway, fatty acid metabolism, leucine metabolic pathway and high expression of β-globin in response to Aeromonas hydrophila infection Sexual mechanisms to resist the invasion of pathogens. The research results provide important theoretical basis for the subsequent in-depth disclosure of the molecular mechanism of fish disease interaction and the breeding of new species of croaker fish disease resistance.