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目的：研究兔心局部缺血“预处理”后，局部缺血和相邻非缺血心肌热休克蛋白７０（ＨＳＰ７０）ｍＲＮＡ的水平变化。方法：提取心肌组织总ＲＮＡ，用ＨＳＰ７０探针进行斑点杂交。结果：缺血预处理组的缺血区和非缺血区ＨＳＰ７０ｍＲＮＡ表达水平均显著高于对照组相应部位（Ｐ＜００５），而每组内的缺血区及非缺血区间无显著差异（Ｐ＞００５）。结论：局部缺血“预处理”后，缺血和非缺血区两处心肌的内反应呈“一致性”；缺血预处理对心肌的保护作用可能与ＨＳＰ７０的合成增多有关。
Objective: To study the changes of HSP70 mRNA in ischemic and adjacent non-ischemic myocardium after preconditioning of ischemic myocardium in rabbits. Methods: Total RNA was extracted from myocardium and blotted with HSP70 probe. Results: The expression of HSP70 mRNA in ischemic preconditioning group and non-ischemic area was significantly higher than that in control group (P <005), while there was no significant difference in ischemic area and non-ischemic area Difference (P> 005). CONCLUSION: After ischemic “pretreatment”, the internal responses of ischemic myocardium and non-ischemic myocardium are “consistent”. The protective effects of ischemic preconditioning on myocardium may be related to the increased synthesis of HSP70.