论文部分内容阅读
目的：了解血透患者庚型肝炎病毒（ＨＧＶ）感染率及与其他肝炎病毒重叠感染的情况。方法：收集５７份血透患者血清标本，根据ＨＧＶＲＮＡ保守片段５′非编码区序列，设计了两对引物，采用逆转录巢式ＰＣＲ扩增ＨＧＶＲＮＡ，以及ＥＩＡ法检测ＨＧＶＩｇＧ抗体。同时还检测了ＨＢＶＤＮＡ及ＨＣＶＩｇＧ抗体。结果：５７份标本中，４份ＨＧＶＲＮＡ阳性（７．０％），２份ＨＧＶＩｇＧ抗体阳性（３．５％）。ＨＧＶＲＮＡ与ＨＧＶＩｇＧ抗体呈“分离”现象。４份ＨＧＶＲＮＡ阳性标本中２份为ＨＢＶＤＮＡ阳性，２份为ＨＣＶＩｇＧ抗体阳性；其中１份标本ＨＢＶＤＮＡ和ＨＣＶＩｇＧ抗体均为阳性。结论：血透患者ＨＧＶ感染率高于健康献血员和正常人群，ＨＧＶ可与ＨＢＶ和ＨＣＶ重叠感染。
Objective: To understand the infection rate of Hepatitis G virus (HGV) in hemodialysis patients and the overlap with other hepatitis viruses. Methods: Serum samples from 57 patients with hemodialysis were collected. Two pairs of primers were designed according to the 5 ’untranslated region of HGV RNA. HGV RNA was amplified by reverse transcription nested PCR and detected by EIA. Also detected HBVDNA and HCVIgG antibodies. Results: Of 57 specimens, 4 were HGV RNA positive (7.0%) and 2 were HGVIgG positive (3.5%). HGVRNA and HGVIgG antibodies were “isolated” phenomenon. Two of the four HGV RNA positive samples were positive for HBVDNA and two were positive for HCVIgG antibody. One of the samples was positive for both HBVDNA and HCVIgG antibodies. Conclusion: HGV infection rate in hemodialysis patients is higher than that in healthy blood donors and normal people, and HGV can overlap with HBV and HCV infection.