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目的:缺氧是乳腺癌常见的现象,并可能诱导肿瘤的进展。雌激素受体(ER)-α状态是乳腺癌预后和内分泌治疗疗效的重要预测指标。本研究旨在揭示乳腺癌中缺氧与ER-α表达的关系。方法:51例患者经配体结合法证实为ER阳性的乳腺癌。采用免疫组织化学染色的方法观察ER-α的异质性表达与多种缺氧标志的关系。结果:免疫组织化学染色发现本组有49例乳腺癌为ER-α阳性。无论乳腺导管内癌(n=29)还是浸润性癌(n=20),ER-α蛋白在邻近坏死灶的区域都出现了下降(P分别≤0.0001);ER-α的区域性下调还与缺氧诱导基因CA-Ⅸ和Glut-1的表达有关(P<0.0001)。结论:乳腺癌中ER-α区域性的表达下降与缺氧有关。因此缺氧可能促使乳腺癌在进展过程中向ER-α阴性的表型演变。
Purpose: Hypoxia is a common phenomenon in breast cancer and may induce tumor progression. Estrogen receptor (ER) -α status is an important prognostic indicator of breast cancer prognosis and endocrine therapy. This study aimed to reveal the relationship between hypoxia and the expression of ER-α in breast cancer. Methods: Fifty-one patients with ER-positive breast cancer were confirmed by ligand binding assay. Immunohistochemical staining was used to observe the relationship between the heterogeneous expression of ER-α and various hypoxia markers. Results: Immunohistochemical staining found that 49 cases of breast cancer in this group were positive for ER-α. ER-α protein decreased in the area adjacent to the necrotic lesion (both P = 0.0001), regardless of intraductal carcinomas (n = 29) or invasive carcinomas (n = 20). The regional downregulation of ER-α was also associated with Hypoxia-inducible gene CA-Ⅸ and Glut-1 expression (P <0.0001). Conclusion: The decreased expression of ER-α in breast cancer is related to hypoxia. Therefore, hypoxia may promote breast cancer progression to ER-α-negative phenotype.