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在离体条件下研究盐胁迫对二倍体马铃薯富利亚(Solanum phureja,PHU)和窄刀薯(S.stenotomum,STN)杂种(PHU-STN)无性系叶绿素含量和抗氧化酶活性的影响,以期为二倍体马铃薯耐盐性鉴定与评价提供理论依据。根据盐胁迫下6项生长参数的表现建立耐盐程度不同的3个组:5份耐盐无性系、5份中耐盐无性系、5份感盐无性系。以四倍体品种Bintje为耐盐对照,Mainechip为感盐对照,用0(CK)、10、20和30mmol/L的NaCl进行胁迫,测定叶绿素含量、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase,POD)活性的变化。结果表明,随着盐浓度的升高,耐盐组叶绿素含量相对值呈先升高后降低的趋势,中耐盐组和感盐组则呈降低的趋势,二倍体组间差异极显著;耐盐组的SOD活性相对值呈升高的趋势,中耐盐组和感盐组呈先降低后升高的趋势,二倍体组间差异极显著;耐盐组和中耐盐组POD活性相对值呈先降低后升高的趋势,感盐组呈降低的趋势,二倍体组间差异极显著。因此,叶绿素含量、SOD和POD活性可以作为二倍体马铃薯耐盐性鉴定的间接指标。
Effects of Salt Stress on Chlorophyll Content and Activity of Antioxidant Enzymes in Two Somatic Hybrid Rice Solanum Phurea (PHU) and S. stenotomum (STN) Hybrids (PHU-STN) in Vitro , In order to provide a theoretical basis for identification and evaluation of salt tolerance of diploid potato. According to the performance of six growth parameters under salt stress, three groups with different salt tolerance were established: 5 salt tolerant clones, 5 medium salt tolerant clones and 5 salt sensitive clones. The tetraploid variety Bintje was used as salt tolerance control. Mainechip was used as a salt sensitive control. The chlorophyll content, superoxide dismutase (SOD) and chlorophyll content were determined by 0, 10, 20 and 30 mmol / And peroxidase (POD) activity changes. The results showed that with the increase of salt concentration, the relative value of chlorophyll content of salt tolerant group first increased and then decreased, while that of medium salt tolerant group and salt sensitive group tended to decrease. The difference between the two groups was extremely significant. The relative value of SOD activity in salt-tolerant group tended to increase, while the salt-tolerant and salt-tolerant groups tended to decrease firstly and then increase, and the difference between diploid groups was extremely significant. The activity of POD The relative value showed a trend of decreasing firstly and then increasing. Salt-sensitive group showed a decreasing tendency, and the difference between diploid groups was extremely significant. Therefore, chlorophyll content, SOD and POD activities can be used as indirect indicators of salt tolerance of diploid potato.