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细胞学特征的研究是解析小麦光温敏雄性不育机理的重要基础,已有研究表明小麦光温敏核雄性不育系BS366的败育可能和细胞骨架异常相关。但是与微管骨架观察相比,通常微丝骨架荧光标记难度大、效果欠佳。通过对比和分析利用FAA固定液和多聚甲醛固定液固定材料的微丝TRITC-phalloidin荧光标记,结果表明利用FAA固定液固定花药组织进行微丝标记的形态效果较好,进一步用植物Actin单克隆抗体做间接免疫荧光标记验证了FAA固定材料的可靠性。试验为研究小麦光温敏雄性不育系减数分裂期微丝行为提供了更简便有效的技术和方法,同时也对其他高等植物花粉母细胞的微丝骨架荧光标记观察具有一定的借鉴作用。
The study of cytological characteristics is an important basis for resolving the mechanism of photo-thermo-sensitive male sterility in wheat. It has been shown that the abortion of the photo-thermo-sensitive genic male sterile line BS366 may be related to the cytoskeleton anomaly. However, compared with the observation of the microtubule structure, usually the microfilament skeleton fluorescence labeling is difficult, the effect is not good. By comparing and analyzing the fluorescence labeling of TRITC-phalloidin using FAA fixative and paraformaldehyde fixative, the results showed that the morphological effect of immobilizing the anther tissue with FAA fixative was better, and then the plant Actin monoclonal Indirect immunofluorescence labeling of antibodies validated the reliability of the FAA fixatives. The experiment provided a simple and effective technique and method for studying the meiosis behavior of the photosynthetic male sterile lines of wheat at the meiosis stage, and also had some reference effects on the fluorescent labeling of the actin filaments of other higher plant pollen mother cells.