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目的 研究应用荧光原位杂交 (FISH)方法预测人肿瘤细胞放射敏感性及其应用的可行性。方法 用 3种放射敏感性不同的人肿瘤细胞株 [鼻咽鳞癌 (CNE)、肺腺癌 (SPC)和乳腺癌 (MCF 7) ],常规克隆形成方法测定不同剂量照射后的存活分数和照射后 2 4h经秋水仙素阻断细胞分裂周期 ,低渗、固定、常规染色体制片后 ,采用 8号染色体涂染探针和FISH方法测定肿瘤细胞 8号染色体诱导的畸变量。结果 2、4、6Gy照射后 2 4h ,CNE、SPC和MCF 7细胞诱导生成的残存染色体畸变能够反映细胞的放射敏感性 ,所有细胞株诱导染色体畸变与细胞存活分数分别存在良好相关性(r=0 .98) ,3种细胞株SF2和相应残存染色体畸变也存在良好相关性 (r=0 .96 )。结论 采用FISH方法计数照射诱导的残存染色体畸变 ,可以预测肿瘤细胞的放射敏感性差异并具有重要的临床意义
Objective To study the feasibility of using fluorescence in situ hybridization (FISH) to predict the radiosensitivity of human tumor cells and its feasibility. Methods Three kinds of human tumor cell lines with different radiosensitivity (nasopharyngeal squamous cell carcinoma (CNE), lung adenocarcinoma (SPC) and breast carcinoma (MCF 7) ), conventional colony formation method were used to determine the survival fraction after different doses of irradiation. After 24 hours of irradiation, the cell division cycle was blocked by colchicine, and hypotonic, fixed, and conventional chromosome production was used. The chromosome 8 induced distorted variable was detected by chromosome 8 staining probe and FISH method. Results The residual chromosomal aberrations induced by CNE, SPC and MCF 7 cells could reflect the radiosensitivity of the cells 24 h after 2, 4 and 6 Gy irradiation. There was a good correlation between chromosome aberrations and cell survival scores in all cell lines (r= In 0.98), there was also a good correlation between SF2 and the corresponding residual chromosomal aberrations (r=0.96). Conclusion The use of FISH to count residual chromosomal aberrations induced by irradiation can predict the differences in radiosensitivity of tumor cells and have important clinical significance.