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随着世界人口的迅速增长,人类对粮食的需要量日益增加,以及由于能源危机使氮肥生产供不应求和成本增加,因而对生物固氮的研究日益受到人们的重视,并就生物固氮的各个方面开展了广泛的研究,取得了迅速的进展。就测定生物固氮的方法来说,自1883年开始采用凯氏定氮法测定生物在 N_2中生长前后的总氮差值确定固氮量以来;随着研究固氮的深入和发展,已提出和试验过了各种测定固氮的方法。
With the rapid growth of the world’s population, increasing human needs for food and the shortage of nitrogen fertilizer production due to the energy crisis, the research on biological nitrogen fixation has drawn more and more attention and carried out various aspects of biological nitrogen fixation Extensive research has made rapid progress. For the determination of biological nitrogen fixation method, the determination of nitrogen fixation by Kjeldahl method since 1883 was used to determine the difference in total nitrogen between before and after growth in N 2. With the deepening and development of research on nitrogen fixation, it has been proposed and tested A variety of methods for determining nitrogen fixation.