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目的:通过观察四生汤对生大黄致脾虚证小鼠一般生活状况、免疫功能、抗应激能力的影响,探讨其治疗脾虚证的作用机制。方法:将小鼠按照体质量随机分为正常组、脾虚模型组和低、中、高剂量四生汤组。采用造模与治疗同步的方式,即上午治疗组分别予高、中、低三个剂量的四生汤灌胃,脾虚模型组及正常组给予蒸馏水灌胃;下午除正常组予蒸馏水外,其余组给予生大黄粉灌胃,连续给药14d。观察小鼠的一般生活状况,并检测肛温、负重游泳时间、胸腺指数、脾脏指数和血清白细胞介素2(interleukin-2,IL-2)的含量。结果:灌服四生汤的小鼠较脾虚模型组精神好,稀便减少,食欲增强,体质量增长快;高、中剂量四生汤能明显提高脾虚证小鼠的肛温,改善畏冷;高、中、低剂量四生汤组的脾脏指数和胸腺指数与正常组比较差异无统计学意义(P>0.05),而胸腺指数较脾虚模型组升高(P<0.05)。高、中剂量的四生汤能明显提高脾虚证小鼠血清IL-2的含量(P<0.05),明显延长脾虚证小鼠的负重游泳时间(P<0.05)。结论:高、中剂量的四生汤具有良好的健脾益气作用,提高机体的免疫功能和抗应激能力是其主要的作用机制。
Objective: To observe the effect of Sshengtang decoction on the general living conditions, immune function and anti-stress ability in mice induced by rhubarb-induced spleen deficiency syndrome and to explore its mechanism of action in treating spleen deficiency syndrome. Methods: According to body weight, mice were randomly divided into normal group, spleen deficiency model group and low, medium and high dose Ssangtang group. The model and treatment were synchronized, that is, the morning treatment group were given high, medium and low doses of three Sansheng Tang gavage, spleen deficiency model group and normal group given distilled water; afternoon except normal group with distilled water, the rest Group given rhubarb powder gavage, continuous administration 14d. The general living condition of mice was observed. The rectal temperature, weight-bearing swimming time, thymus index, spleen index and serum interleukin-2 (IL-2) Results: Compared with spleen-deficiency model group, the mice fed with Sisheng Decoction had better spleen, reduced stool loss, increased appetite and faster body weight increase. High and medium doses of Ssang-Sang Decoction could significantly improve rectal temperature and amelioration There was no significant difference in spleen index and thymus index between the high, middle and low doses of Ssang Sang Decoction group and the normal group (P> 0.05), but the thymus index was higher than that of the spleen deficiency model group (P <0.05). High and medium doses of Ssang-Sang Decoction could significantly increase the level of IL-2 in spleen deficiency mice (P <0.05), and prolong the time of weight-bearing swimming in spleen deficiency mice (P <0.05). Conclusion: The high and middle dose of Ssang Sang Decoction has the good effect of invigorating the spleen and invigorating Qi, and its main function mechanism is to improve the body’s immune function and anti-stress ability.