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目的研究龙牙楤木体细胞胚发生、发育及植株再生过程中,胚性愈伤组织、球形胚、子叶胚、体胚苗的生长和其主要药用成分总皂苷、齐墩果酸、总黄酮的累积情况。方法采用组织培养诱导体细胞胚发生,测量体细胞胚发育过程中各发育时期的生物生长量,并用分光光度法分析其中的总皂苷和总黄酮的量,采用高效液相法测定齐墩果酸的量。结果随着胚性愈伤组织的分化、发育,其生长速率由胚性愈伤组织的每周0.09 g,递增到体细胞胚苗的每周0.32 g。胚性愈伤组织和各不同发育时期体胚中总皂苷和齐墩果酸量的趋势相同,即体胚苗>球形体胚>胚性愈伤组织>子叶体细胞胚;与总黄酮的量不同,即体胚苗>子叶体细胞胚>胚性愈伤组织>球形体胚。结论胚性愈伤组织和各不同发育时期体胚中,总皂苷、齐墩果酸、总黄酮的生长速率最高的是体胚苗,分别为每周6.72、3.42、7.36 mg。
Objective To study the growth and development of embryogenic callus, globular embryo, cotyledonary embryo and somatic embryo in the process of the development, development and plant regeneration of Alobum mongolicum somatic embryogenesis and its main medicinal components of total saponin, oleanolic acid, total The accumulation of flavonoids. Methods The somatic embryogenesis was induced by tissue culture to measure the growth of somatic embryos in different developmental stages. The total saponins and total flavonoids were determined by spectrophotometry. The content of oleanolic acid The amount of. Results With the differentiation and development of embryogenic callus, its growth rate increased from 0.09 g per week of embryogenic callus to 0.32 g per week of somatic embryo. The trend of total saponin and oleanolic acid in embryogenic callus and somatic embryos in different developmental stages were the same, that is, somatic embryos> globular somatic embryos> embryogenic callus> cotyledons somatic embryos; Different, that is, somatic embryos> cotyledons somatic embryos> embryogenic callus> spherical somatic embryos. Conclusion The highest growth rate of total saponin, oleanolic acid and total flavonoids in embryogenic callus and somatic embryos at different developmental stages are somatic embryos, 6.72, 3.42 and 7.36 mg per week, respectively.