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为了解落地生根(Kalanchoe daigremontiana)的SAHH基因功能,采用RACE技术从其叶片克隆了SAHH的全长c DNA序列,命名为Kd SAHH。结果表明,Kd SAHH全长为1748 bp,含1458 bp完整的开放阅读框(ORF),推测编码485个氨基酸。预测落地生根SAHH蛋白分子量约为53 k Da,理论等电点为5.59~5.682。Scanprostie和DNAstar预测表明,落地生根SAHH蛋白在进化上非常保守,与苜蓿的亲缘关系较近。以黄羽扇豆为模板,利用SWISS-MODLE和Phyre程序模拟的落地生根SAHH蛋白亚基三维结构有一定差异。这些为落地生根Kd SAHH的表达和功能研究奠定了基础。
In order to understand the function of SAHH gene in Kalanchoe daigremontiana, the full-length c DNA sequence of SAHH was cloned from its leaves by RACE technique and named Kd SAHH. The results showed that the full length of Kd SAHH was 1748 bp, containing a complete open reading frame (ORF) of 1458 bp, which was deduced to encode 485 amino acids. The predicted SAHH protein molecular weight was about 53 kDa and the theoretical isoelectric point was 5.59-5.682. Scanprostie and DNAstar predictions showed that the SAHH protein from rooting is evolutionarily conserved and closely related to alfalfa. Using yellow lupine as a template, the three-dimensional structure of SAHH protein subunits in rooting plants simulated by SWISS-MODLE and Phyre programs were different. These laid the foundation for the study on the expression and function of Kd SAHH.