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目的:研究腋淋巴结阴性乳腺癌(ALNNBC)血管内皮生长因子(VEGF)血清浓度与乳腺癌微转移关系。方法:采用巢式RT-PCR方法,检测62例ALNNBC外周血人类乳腺珠蛋白(hMAM)mRNA的表达,阳性表达者判定为乳腺癌微转移;ELISA方法检测患者VEGF血清浓度。分析hMAM mRNA表达及VEGF血清浓度与相关临床病理因素间的关系,VEGF血清浓度与微转移及复发转移的关系。结果:ALNNBC中c-erbB2阳性表达者hMAM mRNA阳性表达率高(P<0.05);hMAM mRNA阳性表达提示乳腺癌存在微转移;ER阴性、P53阳性、c-erbB2阳性、hMAM mRNA阳性表达组VEGF血清浓度高(P<0.05或者P<0.01);高VEGF浓度组无复发生存率较低(P<0.05)。结论:ALNNBC外周血血清VEGF高浓度与微转移及临床复发和转移密切相关,血清VEGF浓度可能是ALNNBC预后预测因子。
Objective: To investigate the relationship between serum VEGF level and the micrometastasis in breast cancer with axillary lymph node-negative breast cancer (ALNNBC). Methods: The expression of hMAM mRNA in peripheral blood of 62 patients with ALNNBC was detected by nested RT-PCR. The positive expression of hMAM mRNA was detected as micro-metastasis of breast cancer. ELISA was used to detect the serum concentration of VEGF. To analyze the relationship between hMAM mRNA expression and VEGF serum concentration and related clinicopathological factors, the relationship between VEGF serum concentration and micrometastasis and recurrence and metastasis. Results: The positive expression rate of hMAM mRNA in c-erbB2-positive ALNNBC was significantly higher than that in c-erbB2-positive (P <0.05). The positive expression of hMAM mRNA in breast cancer showed that there was micrometastasis. ER, P53, c- Serum concentration was higher (P <0.05 or P <0.01). The recurrence-free survival rate in high VEGF concentration group was lower (P <0.05). Conclusion: The high concentration of serum VEGF in peripheral blood of ALNNBC is closely related to micrometastasis and clinical recurrence and metastasis. Serum VEGF concentration may be a prognostic factor of ALNNBC prognosis.