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目的报道1例睾丸完全不运动精子经冷冻-解冻后应用激光筛选存活精子行卵胞浆内单精子注射(ICSI)获得临床妊娠。方法睾丸穿刺获取的完全不运动精子经激光鉴定有存活的精子,立即进行冷冻保存。取卵日,解冻该精子应用激光选择存活的精子进行卵胞浆内单精子注射。结果睾丸完全不运动精子冷冻前和解冻后经激光鉴定存活率分别为55.75%和39.81%。选择激光鉴定为存活的精子注射到5个成熟卵母细胞中,4个卵子正常受精(受精率80%),培养至第3天均卵裂(卵裂率100%),移植两枚优质胚胎后获得临床妊娠。结论完全不运动精子是可以冷冻保存的。
Objective To report a case of testicular complete non-exercise sperm frozen-thawed by laser screening of surviving sperm intracytoplasmic sperm injection (ICSI) for clinical pregnancy. Methods Totally non-motile spermatozoa obtained by testicular sperm were identified by laser sperm survival, cryopreservation immediately. Ovulation day, thaw the sperm application of laser selection survival of sperm intracytoplasmic sperm injection. Results The testicles were completely inactive. The pre-frozen and post-thawed sperm motifs were 55.75% and 39.81%, respectively. Four sperm were selected and fertilized. Four oocytes were fertilized normally (fertilization rate 80%), cultured to the third day with cleavage (cleavage rate 100%). Two high quality embryos were transplanted After getting clinical pregnancy. Conclusion No exercise sperm can be stored frozen.