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目的:观察补中益气汤对吗啡戒断大鼠前列腺指数和血清性激素的影响。方法:用40只成年♂Wistar大鼠皮下定量递增注射吗啡5d,建立吗啡依赖大鼠模型。d6停止注射吗啡,观察大鼠的戒断反应。自d7开始,除正常对照组和吗啡戒断组外,实验组每天分别给予不同剂量的补中益气汤(2.5,5,10ml·kg-1,ig)。5周后测定大鼠血清睾酮和雌激素水平;摘取前列腺,称体重及前列腺湿重,计算前列腺指数。结果:不同剂量的补中益气汤明显降低吗啡戒断大鼠前列腺湿重、前列腺指数和血清睾酮水平,有升高大鼠血清雌二醇的作用,中剂量(5ml·kg-1)和大剂量(10m·lkg-1)作用明显(P<0.01)。结论:补中益气汤可明显降低吗啡戒断大鼠前列腺指数,且呈剂量依赖关系;明显降低吗啡戒断大鼠血清睾酮水平,有升高大鼠血清雌二醇的作用。补中益气汤抑制吗啡戒断大鼠前列腺组织增生的作用与血清性激素水平变化有关。
Objective: To observe the effect of Buzhong Yiqi Decoction on prostate index and serum sex hormone in morphine withdrawal rats. METHODS: Morphine-dependent rats were established in 40 adult Wistar rats by injecting morphine into the skin subcutaneously for 5 days. The injection of morphine was stopped on d6 and the withdrawal response of the rats was observed. Starting from d7, in addition to the normal control group and the morphine withdrawal group, the experimental group was given different doses of Buzhong Yiqi Decoction (2.5, 5, 10 ml·kg-1, ig) each day. After 5 weeks, the serum testosterone and estrogen levels were measured; the prostate was removed, the body weight and the wet weight of the prostate were calculated, and the prostate index was calculated. RESULTS: Buzhong Yiqi Decoction with different doses significantly reduced the prostatic wet weight, prostate index, and serum testosterone levels in morphine withdrawal rats, and increased serum estradiol level in rats, with medium dose (5 ml·kg-1) and large. The effect of dose (10m·lkg-1) was obvious (P<0.01). Conclusion: Buzhong Yiqi Decoction can significantly reduce the prostate index of morphine withdrawal rats in a dose-dependent manner. The serum testosterone level in morphine withdrawal rats can be significantly decreased, and the serum estradiol level can be increased. The effect of Buzhong Yiqi Decoction on the inhibition of prostatic hyperplasia in morphine withdrawal rats is related to the change of serum sex hormone levels.