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观察维甲酸(RA)的抗纤维化作用并初步探讨其作用机理。RA治疗博莱霉所致肺纤维化大鼠28天后,病理观察及形态定量分析示肺纤维化程度明显减轻,肺组织胶原蛋白含量较同期纤维化对照组(BLM组)减少(113.86±13.86mg/glung;152.40±11.00mg/glung,P<0.01)。RA组支气管肺泡灌洗液中转化因子β1(TGF-β1)含量在第7、14天时则较同期BLM组显著下降(P均<0.01)。RA治疗后,肺组织原胶原α1(Ⅰ).(Ⅲ)及TGF-β1.mRNA水平也有明显的降低。RA能有效阻止实验性肺间质纤维化的发生和发展。RA抑制肺组织TGFβ1的表达是其抗纤维化作用的重要机制之一。
To observe the anti-fibrosis effect of retinoic acid (RA) and to explore its mechanism. After 28 days of RA treatment of bleomycin-induced pulmonary fibrosis in rats, pathological observation and morphometric analysis showed that the degree of pulmonary fibrosis was significantly reduced, and the content of collagen in lung tissue was decreased compared with the BLM group (113.86 ± 13.86 mg / glu; 152.40 ± 11.00 mg / glung, P <0.01). The content of transforming growth factor-β1 (TGF-β1) in bronchoalveolar lavage fluid of RA group was significantly lower than that of BLM group on the 7th and 14th days (all P <0.01). After RA treatment, lung tissue procollagen α1 (Ⅰ). (Ⅲ) and TGF-β1. mRNA levels also significantly reduced. RA can effectively prevent the occurrence and development of experimental pulmonary fibrosis. Inhibition of RA expression of TGFβ1 in lung tissue is one of the important mechanisms of anti-fibrosis.