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本文应用抗A原体和B寡体单克隆抗体(McAb)分析其抗原表位在对百日咳免疫中所起的作用。方法是以去毒百日咳毒素(Pгd)免疫小鼠,取脾细胞与P3U1骨髓瘤细胞按常规方法融合,采用酶免疫分析法(EIA)筛选阳性杂交瘤上清,从中选出3株(A4、A12、B9)进行免疫化学、生物活性等方面的研究。体外百日咳毒素(PT)活性测定用中国仓鼠卵巢(CHO)细胞集落试验和血凝试验(HA)。McAb体外中和PT活性试验用CHO抑制(CHO-I)和HA抑制(HA-I)法。体内PT活性中和试验在5～8周龄雌性CFW小鼠中进
In this paper, the use of anti-A and B oligomer monoclonal antibodies (McAb) analysis of its antigenic epitopes in the role of pertussis immunity. The method comprises the following steps: immunizing mice with detoxified pertussis toxin (Pгd), taking spleen cells fused with P3U1 myeloma cells by conventional methods, screening the positive hybridoma supernatant by enzyme immunoassay (EIA), selecting 3 strains (A4, A12, B9) for immunochemical, biological activity and other aspects of research. In vitro pertussis toxin (PT) activity assay Chinese hamster ovary (CHO) cell colony assay and hemagglutination assay (HA) were used. In vitro neutralization of McAb PT activity was tested using CHO inhibition (CHO-I) and HA inhibition (HA-I). In Vivo PT Active Neutralization Assay In female CFW mice aged 5-8 weeks