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目的:探讨生长抑素类似物奥曲肽对胰腺癌细胞增殖、凋亡和血管内皮生长因子(VEGF)表达的影响。方法:分别加入不同浓度奥曲肽培养细胞24 h,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖,原位末端转移酶标记法(TUNEL)检测细胞凋亡,实时荧光定量PCR(RT-PCR)法检测细胞VEGF mRNA表达。结果:随着奥曲肽浓度的升高,细胞增殖受到抑制,凋亡率升高,VEGFmNRA表达下降,与对照组比有显著性差异(P<0.05)。结论:奥曲肽可抑制胰腺癌细胞增殖、促进胰腺癌细胞凋亡和降低胰腺癌细胞VEGF的表达。
Objective: To investigate the effect of somatostatin analogue octreotide on proliferation, apoptosis and expression of vascular endothelial growth factor (VEGF) in pancreatic cancer cells. Methods: The cultured cells were treated with different concentrations of octreotide for 24 h, respectively. Cell proliferation was detected by MTT assay. Apoptotic cells were detected by TUNEL. Real-time PCR (RT- PCR) was used to detect the expression of VEGF mRNA. Results: With the increase of octreotide concentration, the cell proliferation was inhibited and the apoptosis rate was increased. The expression of VEGFmNRA was decreased, which was significantly different from the control group (P <0.05). Conclusion: Octreotide can inhibit the proliferation of pancreatic cancer cells, promote the apoptosis of pancreatic cancer cells and reduce the expression of VEGF in pancreatic cancer cells.