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应用AFLP方法,从64对引物中筛选出5对用于分析胡子鲇(Claris fuscus)厦门(XM)、江门(JM)、玉林(YL)等3个养殖群体共90个个体群体内、群体间遗传多样性。扩增共得到289条清晰谱带,引物多态性介于47.8%～63.8%,平均多态性比率为56.4%。胡子鲇群体总遗传杂合度(Ht)、遗传分化系数(Hs)、遗传分化度(Gst)和基因流(Nm)分别为0.161 1、0.153 6、0.051 1和8.501 8,表明胡子鲇多态性处于中等偏下水平,群体内部遗传分化系数较高,三个群体间无较大分化,群体间基因流动较好。其中,JM群体多态性比率、有效等位基因数Ne、Nei’s指数和Shannon指数都较XM群体和YL群体高,种内分化程度较好。对群体进行两两比较发现,XM群体和YL群体间分化程度最大,XM群体和JM群体次之,JM群体和YL群体最小。基于杂交优势,推断XM和YL群体繁殖所产生的后代将具有比亲代更为优良的性状。
Five AFLP primers were screened out from 64 pairs of primers for analysis of 90 individuals from 3 breeding populations including Claris fuscus Xiamen, XM and YL, Genetic diversity. A total of 289 clear bands were obtained. The primer polymorphisms ranged from 47.8% to 63.8% with an average of 56.4%. The total genetic heterozygosity (Ht), genetic differentiation coefficient (Hs), genetic differentiation (Gst) and gene flow (Nm) of Bearded Chinese goose were 0.161, 1,0.153 6, 0.051 1 and 8.501 8, respectively, At the middle-lower level, the coefficient of genetic differentiation was higher in the population, with no significant differentiation among the three populations and better gene flow among the populations. Among them, JM population polymorphism ratio, effective allele number Ne, Nei’s index and Shannon index were higher than those of XM population and YL population, and their intraspecific differentiation was better. The pairwise comparisons of groups showed that the differentiation between XM group and YL group was the largest, followed by XM group and JM group, JM group and YL group were the smallest. Based on the advantages of hybridization, it is inferred that offspring resulting from the multiplication of XM and YL populations will have better traits than their parents.