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目的观察摄食地沟油对小鼠肝肾功能及脂肪代谢的影响。方法将72只小鼠平均分为6组,其中地沟油组与食用油组各3组,按照5、10和20μL/g剂量的地沟油或食用油连续隔天给予小鼠灌胃8周,比较两组小鼠灌胃前后丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、血尿素氮(BUN)、血肌酐(Scr)、甘油三酯(TG)和总胆固醇(TC)水平。结果地沟油的水分含量、酸价和过氧化值指标均超标。连续灌胃8周后,5μL/g剂量地沟油组小鼠ALT明显高于食用油组(P<0.05);10μL/g剂量地沟油组小鼠AST高于食用油组(P<0.05)。5、10和20μL/g剂量地沟油组小鼠Scr均高于同剂量食用油组(P<0.05);10和20μL/g剂量地沟油组小鼠BUN均高于同剂量食用油组(P<0.05);但相同剂量地沟油和食用油组小鼠的TG和TC未见统计学差异(P>0.05)。结论连续8周摄食地沟油对小鼠肝、肾功能均有明显损害作用。
Objective To observe the effects of feeding waste oil on liver and kidney function and fat metabolism in mice. Methods 72 mice were equally divided into 6 groups, of which 3 groups were treated with cooking oil group and cooking oil group respectively. The mice were given gavage oil or cooking oil at doses of 5, 10 and 20 μL / g for 8 weeks. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (Scr), triglyceride Cholesterol (TC) levels. Results The waste oil moisture content, acid value and peroxide value indicators are exceeded. After 8 weeks of continuous gavage, the ALT of mice exposed to 5μL / g ditch oil was significantly higher than that of edible oil (P <0.05). The AST of mice in 10μL / g ditch oil was higher than that of edible oil (P <0.05). The Scr of mice in the waste oil group at 5, 10 and 20 μL / g was higher than that of the same dose of edible oil (P <0.05). The BUN in 10 and 20 μL / g ditch oil group was higher than that of the same dose of edible oil group <0.05). However, no significant difference was found in the TG and TC between the same dosage of waste oil and edible oil group mice (P> 0.05). Conclusion Taken 8 consecutive weeks of waste oil on mice liver and kidney function were significantly damaged.