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目的分析慢性乙型肝炎患者外周血单个核细胞差异表达基因,探索慢性乙型肝炎形成的分子机制。方法应用含14000条人体cDNA的微阵列芯片和来自外周血单个核细胞的标记cDNA,分析了10例慢性乙型肝炎患者和10例健康人基因表达谱。通过应用GenePix4000B扫描仪和ImaGene3.0分析软件比较Cy5标记的慢性乙型肝炎来源cDNA与Cy3标记的健康人来源cDNA的杂交结果,获得个体基因的相对表达比值。结果在分析的14000条基因中,差异表达的基因有92条,占0.66%。其中51条基因表达水平显著上调,41条基因表达水平显著下调。这些差异表达的基因主要为细胞信号转导,细胞周期和代谢,凋亡及炎症相关类基因。结论在乙型肝炎病毒致慢性乙型肝炎过程中,涉及到了众多基因的差异表达,为进一步阐明慢性乙型肝炎形成的分子机制提供基础。
Objective To analyze the differentially expressed genes of peripheral blood mononuclear cells in patients with chronic hepatitis B and to explore the molecular mechanism of chronic hepatitis B formation. Methods The cDNA microarray containing 14000 human cDNAs and the labeled cDNA from peripheral blood mononuclear cells were used to analyze the gene expression profiles of 10 patients with chronic hepatitis B and 10 healthy controls. By using GenePix4000B scanner and ImaGene3.0 analysis software to compare the results of the hybridization between Cy5-labeled chronic hepatitis B-derived cDNA and Cy3-labeled healthy human-derived cDNA, the relative expression ratio of individual genes was obtained. Results Among the 14,000 genes analyzed, 92 differentially expressed genes accounted for 0.66%. Among them, 51 genes were significantly up-regulated and 41 genes were significantly down-regulated. These differentially expressed genes are mainly cell signal transduction, cell cycle and metabolism, apoptosis and inflammation-related genes. Conclusions The differential expression of many genes involved in hepatitis B virus-induced chronic hepatitis B provides the basis for elucidating the molecular mechanism of chronic hepatitis B.