论文部分内容阅读
目的探讨香烟烟雾暴露对大鼠睾丸结构及睾丸组织转铁蛋白(Tf)mRNA及其蛋白表达水平的影响。方法清洁级成年雄性SD大鼠160只,随机分为对照组及低(10根香烟)、中(20根香烟)、高(30根香烟)剂量染毒组,各染毒组大鼠分别染毒2、4、6、8、12周,共16组,每组10只。染毒组大鼠采用呼吸道静式染毒,1次/d,30 min/次。观察大鼠睾丸组织结构,采用RT-PCR及Western blot法检测睾丸组织Tf mRNA及其蛋白表达水平。结果染毒组大鼠睾丸病理切片可见生精上皮层次减少,生精细胞排列疏松,支持细胞空泡化。染毒4、8、12周时高剂量组大鼠睾丸组织Tf mRNA表达水平低于对照组和低剂量组,染毒8周时高剂量组大鼠睾丸组织Tf蛋白表达水平低于对照组,染毒12周时中、高剂量组大鼠睾丸组织Tf蛋白表达水平低于低剂量组,差异均有统计学意义(P<0.05)。结论香烟烟雾暴露可导致雄性大鼠睾丸组织损伤,并抑制Tf基因及蛋白表达。
Objective To investigate the effects of cigarette smoke exposure on the structure of testis and the expression of Tf mRNA and protein in testis. Methods Totally 160 male adult SD rats were randomly divided into control group, low (10 cigarettes), moderate (20 cigarettes) and high (30 cigarettes) dose groups. Rats in each exposure group were dyed Toxic 2,4,6,8,12 weeks, a total of 16 groups of 10. The rats in the exposure group were exposed to the respiratory tract once a day for 30 min every time. The structure of testis in rats was observed. The Tf mRNA and its protein expression in testis were detected by RT-PCR and Western blot. Results The pathological sections of testis showed that the level of seminiferous epithelium decreased, the spermatogenic cells arranged loosely and the supporting cells were vacuolized. At 4, 8 and 12 weeks of exposure, Tf mRNA expression in testis of high-dose group was lower than that in control group and low-dose group. Tf protein expression in testis of high-dose group was lower than that in control group at 8 weeks after exposure. At 12 weeks after exposure, the Tf protein expression levels of testis in middle and high dose groups were lower than those in low dose groups (P <0.05). Conclusion Cigarette smoke exposure can induce the damage of testicular tissue in male rats and inhibit the expression of Tf gene and protein.