论文部分内容阅读
目的研究皖南地区丙型肝炎病毒治疗前后高变区1(HVR1)碱基变异数量与病情变化和治疗效果之间是否具有相关性。方法应用速率法和荧光定量PCR法,检测皖南地区141例慢性丙型肝炎患者血清ALT水平和HCV RNA的载量。应用RT-PCR法进行HCV基因型和治疗前后的HVR1区域序列的检测。结果 141例患者的血清ALT与HCV RNA两指标间采用Spearman等级相关分析显示,rs=0.213,P=0.011,差异具有统计学意义,存在正相关。通过HCV基因1型、2型和未分型3组的HVR1碱基变异数量的单因素方差分析,显示组与组之间差异具有统计学意义。结论慢性丙型肝炎患者治疗前后的HVR1序列碱基变异数量的分析,对治疗效果、病情变化及预后发展等方面的评估具有重要的指导意义。
Objective To investigate whether there is a correlation between the number of base changes in HVR1 and the change of disease and the therapeutic effect before and after hepatitis C virus infection in Southern Anhui Province. Methods The rates of ALT and HCV RNA in serum of 141 patients with chronic hepatitis C in southern Anhui Province were detected by the rate method and the quantitative PCR method. The genotypes of HCV and HVR1 region sequences before and after treatment were detected by RT-PCR. Results The Spearman rank correlation analysis of serum ALT and HCV RNA between 141 patients showed that rs = 0.213, P = 0.011, the difference was statistically significant, there was a positive correlation. One-way ANOVA analysis of the number of base pairs of HVR1 in HCV genotypes 1, 2 and 3 with no genotype showed statistically significant differences between groups. Conclusion The analysis of HVR1 nucleotide variation before and after treatment in patients with chronic hepatitis C has important guiding significance for the assessment of therapeutic effect, disease progression and prognosis.