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实现了葡萄糖氧化酶以及葡萄糖氧化酶和电子传递媒体Fe(CN)_6~(3-)同时在纳米级金膜微带电极上的固定.用红外光谱和循环伏安对GOD/PPy微电极进行了表征,研究了微带金电极上聚吡咯恒电位形成过程的动力学及葡萄糖氧化酶对其动力学过程的影响.探讨了微酶电极GOD/Fe(CN)_6~(3-)/PPy对葡萄糖氧化的催化作用.考察了PPy膜厚度和溶液中氧的存在对GOD/Fe(CN)_6~(3-)/PPy微电极测定葡萄糖的影响。
The immobilization of glucose oxidase, glucose oxidase and Fe (CN) 6 ~ (3-) on the gold nanoparticle was simultaneously performed on the GOD / PPy microelectrodes by infrared spectroscopy and cyclic voltammetry The kinetics of the formation of polypyrrole potentials on gold electrode and the effect of glucose oxidase on its kinetics were investigated. The effects of GOD / Fe (CN) 6 ~ (3 -) / PPy On the glucose oxidation.We investigated the effect of PPy film thickness and oxygen in solution on the determination of glucose by GOD / Fe (CN) _6 ~ (3 -) / PPy microelectrode.