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目的:建立同时测定复方吡拉西坦尼莫地平胶囊中卡托普利、阿司匹林、尼莫地平含量的HPLC法。方法:采用YMC-Pack Pro-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈为流动相A、水(磷酸调节p H至2.5)为流动相B进行梯度洗脱,流速:1.0 ml·min-1,柱温:40℃,检测波长:215 nm(0~4.3 min),276 nm(4.3~11.0 min),235 nm(11.0~18.0 min)。结果:卡托普利、阿司匹林、尼莫地平分别在0.054 7~1.641 8μg(r=0.999 9),0.055 3~1.654 8μg(r=0.999 9),0.077 7~2.331 6μg(r=0.999 7)范围内线性良好,平均加样回收率分别为100.69%(RSD=0.69%,n=6),101.04%(RSD=1.05%,n=6),102.56%(RSD=1.14%,n=6)。结论:该分析方法简便、快速、准确、重复性好,可用于复方吡拉西坦尼莫地平胶囊中卡托普利、阿司匹林、尼莫地平的含量测定。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of captopril, aspirin and nimodipine in Compound Piracetam Nimodipine Capsules. METHODS: The mobile phase B was eluted with a gradient of acetonitrile (acetonitrile, mobile phase A) and water (adjusted to pH 2.5 with phosphoric acid) using a YMC-Pack Pro-C18 column (250 mm × 4.6 mm, 5 μm) · Min-1, column temperature: 40 ℃, detection wavelength: 215 nm (0-4.3 min), 276 nm (4.3-11.0 min) and 235 nm (11.0-18.0 min). RESULTS: Captopril, aspirin, and nimodipine were significantly different from those in the range of 0.054 7 to 1.641 8 μg (r = 0.999 9), 0.055 3 to 1.654 8 μg (r = 0.999 9), 0.077 7 to 2.331 6 μg The average internal standard recoveries were 100.69% (RSD = 0.69%, n = 6), 101.04% (RSD = 1.05%, n = 6) and 102.56% (RSD = 1.14%, n = 6). Conclusion: The method is simple, rapid, accurate and reproducible. It can be used for the determination of captopril, aspirin and nimodipine in Compound Piracetam Nimodipine Capsules.