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目的探讨氟对成人下丘脑-垂体-性腺轴生殖激素水平的影响。方法选择水氟浓度为3.89mg/L的开封孙营村为调查区,另选水氟浓度小于1.0mg/L的沈李楼村作为对照区。对两区居住5年以上全部居民进行健康体检,并测定尿氟含量。随机抽取空腹静脉血150份,应用放射免疫方法测定血清促性腺激素释放激素(GnRH)、促黄体生成素(LH)、睾酮(T)和雌二醇(E2)水平。结果高氟区水氟、土壤氟和粮食氟含量均高于对照区(P<0.05),且高于国家标准;空气氟两地区均未检出。高氟区人均日摄氟量高于对照区(P<0.05),且高于国家标准。高氟区人群尿氟含量明显高于对照区(P<0.05)。高氟区男性、女性血清GnRH水平与对照区比较差异均无显著性。高氟区男性血清LH水平明显高于对照区(P<0.05),女性血清LH水平与对照区比较,差异无显著性。高氟区男性血清T水平明显低于对照区(P<0.05),而女性血清T水平则明显高于对照区(P<0.05)。高氟区男性、女性血清E2水平与对照区比较差异均无显著性。结论氟能影响成人下丘脑-垂体-性腺轴各层面生殖激素水平,因而表现出生殖内分泌干扰作用,且对男性的影响大于女性。
Objective To investigate the effect of fluoride on the level of reproductive hormones in the hypothalamus - pituitary - gonadal axis in adults. Methods Sunye Village, Kaifeng, whose water fluoride concentration was 3.89 mg / L, was selected as the survey area, and Shenlilou Village with an alternative fluoride concentration less than 1.0 mg / L was selected as the control area. All residents living in the two districts for more than 5 years had a healthy physical examination and urine fluoride content was measured. 150 fasting venous blood samples were randomly selected and the serum levels of gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), testosterone (T) and estradiol (E2) were measured by radioimmunoassay. Results The contents of fluoride in water, soil fluoride and food in high fluoride area were higher than those in control area (P <0.05), and higher than national standards. No detection was found in air fluoride area. The per-unit daily fluoride exposure of high fluoride area was higher than that of the control area (P <0.05), and higher than the national standard. Urinary fluoride levels in high fluoride area were significantly higher than those in control area (P <0.05). There was no significant difference in serum GnRH levels between men and women in high fluoride area and control area. The level of serum LH in male high-fluoride area was significantly higher than that in control area (P <0.05). There was no significant difference in female LH serum level between the two groups. The level of serum T in high fluoride area was significantly lower than that in control area (P <0.05), while the level of serum T in female was significantly higher than that in control area (P <0.05). High fluoride area male and female serum E2 levels compared with the control area showed no significant difference. CONCLUSION: Fluoride can affect the level of reproductive hormones in the hypothalamus-pituitary-gonadal axis in adults and thus has reproductive endocrine disrupting effects and its effect on males is greater than that of females.