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1目的 观察 1型血管紧张素 受体 (AT1 R)基因多态性与中国人脑梗死 (CI)和原发性高血压(HTN)的关系 ,并探讨 CI的发病机制。2方法 应用聚合酶链反应、限制性内切酶酶解、电泳分型的方法 ,分析了196例中国人 AT1 R基因中 3′- UTR的 116 6 C变异的多态性分布情况。3结果 CI组中 116 6 A/ 116 6 A,116 6 A/116 6 C,116 6 C/ 116 6 C基因型频率为 0 .5 32 3,0 .30 6 5 ,0 .16 13;HTN组中分别为 0 .5 45 5 ,0 .40 0 0 ,0 .0 5 45 ;对照组中分别为 0 .75 95 ,0 .2 15 2 ,0 .0 2 5 3.CI组基因型频率与对照组比较差异有显著性 (χ2 =11.3992 ,P<0 .0 1) ,HTN组与对照组比较差异也有显著意义 (χ2 =6 .75 93,P<0 .0 5 )。CI组 116 6 C突变频率为 0 .3145 ,HTN组为 0 .2 5 45 ,对照组为 0 .132 9,CI组与对照组比较差异有显著性 (χ2 =13.6 797,P<0 .0 1) ,HTN组与对照组比较差异有显著性(χ2 =6 .42 18,P<0 .0 5 )。女性病人 CI组 116 6 C等位基因频率为 0 .3333,HTN组为 0 .3333,对照组为 0 .10 2 6 ,CI组与对照组比较差异有显著性 (χ2 =11.5 433,P<0 .0 1) ,HTN组与对照组比较差异有显著性 (χ2 =11.16 6 8,P<0 .0 1)。4结论 AT1 R基因的多态性可能参与 CI发病 ,尤其是女性病人 CI的发病。
1 Objective To investigate the relationship between angiotensin-1 type 1 receptor (AT1R) gene polymorphism and cerebral infarction (CI) and essential hypertension (HTN) in Chinese and to explore the pathogenesis of CI. 2 Methods The distribution of 116 6 C polymorphism of 3’-UTR in 196 Chinese patients with AT1 R gene was analyzed by polymerase chain reaction, restriction endonuclease digestion and electrophoresis typing. 3 Results In the CI group, the frequencies of 116 6 A / 116 6 A, 116 6 A / 116 6 C, 116 6 C / 116 6 C genotypes were 0.532 3,0 .30 6 5,0.16 13; HTN The group were 0 .5 455, 0 .40 0 0, 0 .0 5 45; the control group were 0 .75 95, 0 .2 15 2, 0 .0 2 5 3.CI genotype frequency Compared with the control group, the difference was significant (χ2 = 11.3992, P <0.01). There was also significant difference between the HTN group and the control group (χ2 = 6.7593, P <0.05). The mutation frequency of 116 6 C in CI group was 0.3145, in HTN group was 0.5255, in control group was 0.132 9, the difference between CI group and control group was significant (χ2 = 13.6797, P <0. 0 1). There was significant difference between HTN group and control group (χ2 = 6 .42 18, P <0.05). The frequency of allele 116 C in female patients was 0.3333 in CI group, 0.3333 in HTN group and 0.10 26 in control group. There was significant difference between CI group and control group (χ2 = 11.5433, P < 0 .0 1). There was significant difference between HTN group and control group (χ2 = 11.16 6 8, P <0.01). 4 Conclusion AT1 R gene polymorphism may be involved in the pathogenesis of CI, especially in female patients CI.