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为了研究橄榄苦苷对D-半乳糖致衰老小鼠的抗衰老作用及其机制,将40只健康小鼠随机分成正常组、衰老模型组、橄榄苦苷组和维生素E组,采用D-半乳糖建立亚急性衰老小鼠模型,测定每组小鼠的脏器指数,比色法检测心、肝和脑组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、及丙二醛(MDA)含量,PCR-ELISA检测心、肝和脑组织端粒酶活性,ELISA检测小鼠血清中Ig G、Ig M和补体C3、C4含量,放射免疫法测定血清中IL-2含量。结果显示,与模型组比较,橄榄苦苷能增加小鼠脏器指数(P<0.05);提高SOD、GSH-Px活性,降低MDA含量(P<0.05,P<0.01);提高心、肝和脑端粒酶活性、血清中Ig G、Ig M和补体C3、C4及IL-2的含量(P<0.05,P<0.01),上述衰老相关生化指标基本恢复到正常水平,且与维生素E的治疗结果无显著差异(P>0.05)。表明橄榄苦苷能通过拮抗自由基损伤,增强衰老小鼠心、肝和脑组织端粒酶活性和机体免疫功能发挥抗衰老作用。
In order to study the anti-aging effect and mechanism of oleuropein on aging mice induced by D-galactose, 40 healthy mice were randomly divided into normal group, aging model group, oleuropein group and vitamin E group, Lactose to establish a sub-acute aging mouse model. The index of each group of mice was measured. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH- Px activity and MDA content were determined. The telomerase activity in heart, liver and brain was detected by PCR-ELISA. The contents of Ig G, Ig M and C3 and C4 in serum were detected by ELISA. Serum IL-2 content. The results showed that compared with the model group, oleuropein could increase the organ index (P <0.05), increase the activities of SOD and GSH-Px and decrease the content of MDA (P <0.05, P <0.01) (P <0.05, P <0.01). The senescence-related biochemical parameters recovered to normal levels, and the contents of Ig G, Ig M and C3, C4 and IL- There was no significant difference in treatment outcome (P> 0.05). Show that oleuropein can play an anti-aging role by antagonizing free radical damage, enhancing telomerase activity and immune function in the heart, liver and brain of aged mice.