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目的探讨结直肠癌患者血清中肝细胞生长因子(HGF)的表达水平及其与结直肠癌转移和预后的关系,以及其作为结直肠肿瘤标志物的可行性。方法应用夹心ELISA方法检测65例结、直肠癌患者及20例健康志愿者血清HGF,癌胚抗原(CEA),糖抗原(CA19-9)的表达水平;Kap-lan-Meier法计算患者生存率;Cox生存分析血HGF,癌胚抗原(CEA),糖抗原(CA19-9)水平对患者预后的影响。结果结直肠癌淋巴结转移组HGF水平为(0.37±0.11)ng/mL;T3-4期为(0.32±0.29)ng/mL;肝转移组血清HGF水平为(0.43±0.10)ng/mL,上3组均显著高于对照组的(0.18±0.07)ng/mL(P<0.05),亦明显高于各对应组(P<0.05)。单因素分析显示HGF水平越高者生存期越短。结论血清HGF水平与结直肠癌发展及转移有密切关系;结直肠癌患者血HGF水平增高提示患者预后差。HGF可作为检测结、直肠癌转移和判断预后的临床参考指标。
Objective To investigate the expression of hepatocyte growth factor (HGF) in serum of patients with colorectal cancer and its relationship with metastasis and prognosis of colorectal cancer, and its feasibility as a marker for colorectal cancer. Methods Serum HGF, carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) were detected by sandwich ELISA in 65 patients with colorectal cancer and 20 healthy volunteers. The survival rate was calculated by Kap-lan-Meier method. ; Cox survival analysis of blood HGF, carcinoembryonic antigen (CEA), carbohydrate antigen (CA19-9) level on the prognosis of patients. Results The levels of HGF in colorectal cancer lymph node metastasis group were (0.37±0.11) ng/mL, T3-4 stage was (0.32±0.29) ng/mL, and the serum HGF level in liver metastasis group was (0.43±0.10) ng/mL. The three groups were significantly higher than the control group (0.18±0.07) ng/mL (P<0.05), and also significantly higher than the corresponding groups (P<0.05). Univariate analysis showed that the higher the level of HGF, the shorter the survival period. Conclusion The serum level of HGF is closely related to the development and metastasis of colorectal cancer. The increase of serum HGF level in patients with colorectal cancer suggests poor prognosis. HGF can be used as a clinical reference to detect the metastasis of colorectal cancer and prognosis.