目的观察在慢性不可预见性温和应激(chironic unpredictable mild stress,CUMS)下,大鼠下丘脑及杏仁核中促肾上腺皮质素释放激素(corticotropin-releasing hormone,CRH)神经元的激活状态,揭示抑郁症的部分发病机制。方法应用慢性不可预见性的温和刺激建立大鼠抑郁模型。用免疫组化技术观察与应激密切相关的下丘脑室旁核和杏仁核中神经元CRH的表达。结果CUMS大鼠的下丘脑室旁核和杏仁核均有大量的CRH阳性细胞及神经纤维,而对照组CRH阳性神经元很少。结论在抑郁的发生、发展过程中,不仅下丘脑室旁核的CRH神经元起着重要作用,杏仁核的CRH神经元也与应激反应及抑郁症发病机制密切相关。 Objective To observe the activation of corticotropin-releasing hormone (CRH) neurons in rat hypothalamus and amygdala under chronic unpredictable mild stress (CUMS) Part of the pathogenesis of disease. Methods The rat model of depression was established by mild and unpredictable mild stimulation. Immunohistochemistry was used to observe the expression of CRH in neurons in paraventricular hypothalamic nucleus and amygdala, closely related to stress. Results There were a large number of CRH positive cells and nerve fibers in hypothalamic paraventricular nucleus and amygdala in CUMS rats, while there were few CRH positive neurons in CUMS rats. Conclusions CRH neurons in paraventricular nucleus of hypothalamus plays an important role in the process of depression and development. CRH neurons in amygdala are also closely related to stress response and the pathogenesis of depression.