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Ｐ５３基因５—８外显子ＰＣＲ产物的ＤＮＡ序列分析及石蜡包埋组织Ｐ５３鼠抗人单克隆抗体（Ｄｏ－１）免疫组化分析发现：５７例原发性肺癌中３７例有Ｐ５３突变或Ｐ５３蛋白积累。ＤＮＡ／ＰＣＲ产物直接测序发现：９／１２例小细胞肺癌（ＳＣＬＣ），８／１５非小细胞肺癌（ＮＳＣＬＣ）Ｐ５３基因突变，错义突变为１２／１７，Ｇ→Ｔ突变为６／１７。免疫组化发现：１１／１９例ＳＣＬＣ，１９／３７例ＮＳＣＬＣＰ５３蛋白染色阳性。４／２７例经测序方法未发现突变的病例Ｐ５３免疫组化阳性。Ｐ５３基因的异常与肿瘤大小、淋巴结转移及病期无明显相关性。结果提示：Ｐ５３基因的异常可能与原发性肺癌的发病有关，但它不是一个预后指征。
DNA sequence analysis of P53 gene exon 5-8 PCR product and immunohistochemical analysis of paraffin-embedded tissue P53 murine anti-human monoclonal antibody (Do-1) found that 37 out of 57 primary lung cancers had P53 mutations or P53 protein accumulation. Direct sequencing of DNA/PCR products revealed 9/12 cases of small cell lung cancer (SCLC) and 8/15 non-small cell lung cancer (NSCLC) with P53 mutation. The missense mutation was 12/17, and the G→T mutation was 6/17. Immunohistochemical findings: 11/19 cases of SCLC and 19/37 cases of NSCLCP53 stained positive. In 4/27 cases that were not found to have mutations by sequencing, P53 immunohistochemistry was positive. The abnormality of P53 gene had no significant correlation with tumor size, lymph node metastasis and disease stage. The results suggest that the abnormality of P53 gene may be related to the pathogenesis of primary lung cancer, but it is not a prognostic indicator.