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目的:归肾育宫汤是陕西省名中医杨鉴冰教授治疗卵巢早衰的经验方,本文旨在探寻该方对免疫性卵巢早衰小鼠外周血清性激素的影响。方法:60只昆明小鼠,随机分成空白组、模型组、雌激素组、归肾育宫汤大剂量组、归肾育宫汤中剂量组、归肾育宫汤小剂量组,每组10只;除空白组外其余均采用透明带多肽3(2P3)腹腔多点注射复制小鼠卵巢早衰模型,造模成功后,对大、中、小剂量组分别给予归肾育宫汤70g/(kg·d)、35g/(kg·d)、17.5g/(kg·d),雌激素组给予结合雌激素片0.125mg/(kg·d),空白组、模型组给予等量生理盐水,给药4周后,处死大鼠,取血清,检测外周血清性激素卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)水平。结果:模型组小鼠血清FSH、LH显著升高(P<0.05),E2、P明显降低(P<0.05);给药治疗后,雌激素组、归肾育宫汤大、中、小剂量组FSH、LH明显降低(P<0.05),E2、P明显升高(P<0.05),其中以归肾育宫汤大、中最为明显。结论:归肾育宫汤可以降低卵巢早衰大鼠血清卵泡生成素和黄体刺激素的水平,升高雌二醇和孕酮的水平,通过调节外周血性激素水平,对卵巢早衰起到治疗作用。
Objective: Guiyu Gongshen Decoction is a famous doctor in Shaanxi Province Professor Yang Jianbing treatment of premature ovarian failure side of the prescription, this paper aims to explore the side of immune premature ovarian failure in mice serum levels of sex hormones. Methods: Sixty Kunming mice were randomly divided into blank group, model group, estrogen group, Guishen Yugong Decoction high dose group, Guiyu Yugong Decoction middle dose group, Guixin Yugong Decoction small dose group Except for the blank group, all the mice were treated with zona pellucida 3 (2P3) intraperitoneal injection to replicate the mouse model of premature ovarian failure. After the model was established successfully, the rats in the large, medium and small dose groups were given Guiyu Gonggong Decoction 70g / kg · d), 35 g / (kg · d) and 17.5 g / (kg · d) respectively. The estrogen group was given estrogen 0.125 mg / (kg · d) Four weeks after the administration, the rats were sacrificed and serum was taken for detection of serum FSH, LH, E2 and progesterone (P) levels. Results: Serum levels of FSH and LH in model group were significantly increased (P <0.05), and E2 and P were significantly decreased (P <0.05). After administration, estrogen group, Guishen Yugong Decoction large, medium and small dose The levels of FSH and LH were significantly decreased (P <0.05), and E2 and P were significantly increased (P <0.05), among which Guiyu Yugong Decoction was the most obvious. Conclusion: Guiyu Gonggong Decoction can reduce serum levels of serum follicle-stimulating hormone and progesterone in rats with premature ovarian failure, increase the levels of estradiol and progesterone, and regulate the level of serum progesterone in ovary premature ovarian failure.