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本研究旨在探明引起山东部分地区冬小麦苗期生长不良、矮小的原因,并鉴定该病害的病原菌。采用常规组织分离法对小麦水渍状的根系进行分离、纯化,得到2个类似腐霉的分离物SDCY-1和SDJY-1,并对这2个分离物进行形态特征、致病性鉴定及COⅠ序列分析。结果表明,2个分离物菌落白色,菌丝发达,培养基表面有大量的气生菌丝;人工接种后均能引起小麦生长不良、根系变短、鲜重减轻,水渍状至变褐,重新分离后获得的菌株与原接种菌一致。根据形态特征和系统发育树表明2个分离物为同一种腐霉菌—终极腐霉Pythium ultimum。研究结果可为冬小麦腐霉根腐病防治和抗病育种提供科学依据。
The purpose of this study is to identify the causes of poor growth and shortness of winter wheat seedlings in some areas of Shandong and identify the pathogen of the disease. The root tissues of wheat water stains were isolated and purified by conventional tissue separation method. Two kinds of isolates Pythium superoxide dismutase SDCY-1 and SDJY-1 were obtained. Morphological characteristics, pathogenicity identification, COI sequence analysis. The results showed that the colonies of two isolates were white and mycelium was developed with a large number of aerial mycelium on the surface of the culture medium. After inoculation, the growth of the wheat was bad, the root system became shorter, the fresh weight was reduced, Re-isolated strains obtained with the original inoculation of bacteria. According to the morphological characteristics and phylogenetic tree, two isolates were identified as the same Pythium ultimum. The results provide a scientific basis for the prevention and cure of Pythium rot and its resistance in winter wheat.