目的　克隆出视蛋白基因启动子。方法　以小鼠全基因组为模板 ,用PCR法克隆出目的大小的片断。然后连接到T -载体上作酶切鉴定 ,最后测序。结果　酶切结果与预期相符 ,测序结果与公布序列完全一致。结论　视蛋白基因启动子克隆成功 Objective To clone the opsin gene promoter. Methods The whole genome of mice was used as a template to clone the target fragment by PCR. Then ligated to T - vector for restriction enzyme digestion and finally sequenced. Results The results of the digestion were consistent with the expected results. The sequencing results were exactly the same as the published sequence. Conclusion The opsin gene promoter cloning was successful