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目的 研究 Bcl- w蛋白在原发性胆囊癌 (prim arygallbladder carcinoma,PGBC)中的表达及其意义 .方法 以特异性羊抗兔 Bcl- w抗体 ,DAKO Envision TM系统 (L DP法 )免疫组织化学方法检测石蜡包埋的 PGBC标本中 Bcl- w蛋白的表达 .结果 47例 PGBC中 Bcl- w阳性 2 8例 (6 0 % ) ;15例胆囊腺肌瘤样增生 (GBADH) Bcl- w阳性 14例 (93% ) ;Bcl- w在 PGBC中表达低于 GBADH(P<0 .0 5 ) ;Bcl- w在高分化PGBC中的表达高于中低分化 PGBC,其间有显著性差异 (P<0 .0 5 ) ;PGBC中的 Bcl- w表达在肿块体积≤ 2 cm,男性及>5 5岁患者分别高于体积 >2 cm,女性及 <5 5岁患者 ,其间有显著性差异 (P<0 .0 5 ,P<0 .0 5 ,P<0 .0 1) ,但 Bcl- w表达与 PGBC的 Nevin分期无关 .结论 抗凋亡蛋白 Bcl- w参与了 PGBC发病过程的凋亡调变 ,其表达水平的不同与 PGBC临床病情有关 .
Objective To study the expression and significance of Bcl-w protein in primary epithelial gallstone cell carcinoma (PGBC) .Methods Immunohistochemistry with specific goat anti-rabbit Bcl-w antibody and DAKO Envision TM system (L DP method) Methods The expression of Bcl-w protein in paraffin-embedded PGBC specimens was detected.Results The positive expression of Bcl-w in 47 PGBCs was 28 (60%), and 15 cases was positive for Bcl-w in GBADH (93%). The expression of Bcl-w in PGBC was lower than that in GBADH (P <0.05). The expression of Bcl-w in well-differentiated PGBC was higher than that in moderately poorly differentiated PGBC (P < (P> 0.05). The expression of Bcl-w in PGBC was significantly higher than that of tumors with mass less than 2 cm (P <0.05), and was significantly higher in male and> 55 years old than in women> <0.05, P <0.05, P <0.01), but the expression of Bcl-w was not related to the Nevin staging of PGBC.Conclusion The anti-apoptotic protein Bcl-w is involved in the apoptosis of PGBC Change, the different levels of expression and PGBC clinical conditions.