Aristolochic acid (AA) composed of AA-Ⅰ and AA-Ⅱ (Fig.1) is the major toxic component extracted from Aristolochia plants[1]. Recently, it has been proved by several researches that high doses of aristolochic acids (AA) can result in a severe kidney disease named Chinese herbs nephropathy (CHN)[2]. It is characterized by a progressive interstitial fibrosis leading to a severe atrophy of the proximal tubules[3]. In the past several years, CHN has been reported in many countries including Belgium, France, Spain, UK and Japan[1]. Owing to its high toxicity, several health institutions, including the US Food and Drug Administration (FDA), and Medicines Control Agency (MCA), have warned people to pay attention to the safety information about botanical products containing AA. Many countries including UK, Canada, Australia, and Germany have announced banning to restrict the importation, sale and use of AA-containing medicines. So it is of great significance to develop effective and rapid methods to quantify the content of AA in regular herbal medicines.