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目的建立一套简单易操作并在小鼠活体上进行的~(13)C呼气试验检测系统,并探讨国产~(13)C-美沙西丁呼气试验(~(13)C-MBT)对小鼠急性肝损伤的诊断价值。方法~(13)C-美沙西丁经扑热息痛甲烷化合成标记。采用四氯化碳(CCl_4)腹腔注射制作小鼠急性肝损伤模型,造模后饲养1个月复制小鼠急性肝损伤恢复模型,行肝脏病理组织学检查和肝功能血液生化学指标检测,于多个时间点采集活体小鼠~(13)C-美沙西丁灌胃后呼出的气体,以红外线能谱仪(IRIS)检测并绘制呼气峰值(DOB)曲线。结果正常对照组小鼠于灌胃给药后6～8 min时呼气~(13)C达到峰值(51.9±2.04),随后缓慢下降至本底值;模型组于灌胃给药后16 min左右达到峰值(26.37±5.74),随后亦缓慢下降至本底值,两组间差异有统计学意义(P<0.05)。当急性肝损伤恢复模型组和对照组小鼠在相同条件下饲养1个月后,重复~(13)C-美沙西丁呼气检测后两组DOB峰值及达峰时间差异无统计学意义(P>0.05)。结论~(13)C-MBT可在小鼠活体状态下准确、方便地采集和检测呼出气体的~(13)CO_2变化,并正确评估其肝功能的损伤和恢复情况。
Objective To establish a simple and easy-to-operate system of ~ (13) C breath test in vivo of mice and investigate the effect of ~ (13) C-methacetin breath test (~ (13) C-MBT) The diagnostic value of acute liver injury in mice. Method ~ (13) C-methacetin by paracetamol methanation tag. Acute liver injury model was made by intraperitoneal injection of carbon tetrachloride (CCl_4). After the model was established, mice were sacrificed and the model of acute liver injury was replicated for 1 month. Pathological examination of liver and blood biochemical indexes of liver function were performed. The gas exhaled after instillation of ~ (13) C-methacetin in vivo mice was collected at various time points, and the peak expiratory flow (DOB) curve was detected by infrared spectroscopy (IRIS). Results In the normal control group, the exhaled ~ (13) C reached the peak (51.9 ± 2.04) at 6 ~ 8 min after oral administration and then slowly decreased to the background value. In the model group, (26.37 ± 5.74), and then slowly decreased to the background value, the difference between the two groups was statistically significant (P <0.05). When acute liver injury recovery model group and control group mice were kept under the same conditions for 1 month, there was no significant difference in peak and peak DOB levels between the two groups after repeated ~ (13) C-methacetin breath test P> 0.05). Conclusions ~ (13) C-MBT can accurately and conveniently collect and detect the change of ~ (13) CO_2 in exhaled air in mice and correctly evaluate the damage and recovery of liver function.