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目的:探讨Captopril对鼠视网膜新生血管形成的抑制作用。方法:将60只7d龄小鼠随机分为对照组(30只)和治疗组(30只),置于体积分数750±50mL/L高氧环境下饲养5d后回到正常空气环境中饲养,出氧箱后治疗组每天1次玻璃体腔内注射2.7mL/kg Captopril,对照组注射9g/L的氯化钠注射液2.7mL/kg,连续5d。两组小鼠均于17d处死并摘除眼球,采用ADP酶视网膜铺片、HE染色及免疫组织化学法分别观察视网膜血管的改变、计数视网膜新生血管内皮细胞核数及检测MMP-2、PEDF蛋白的表达。结果:治疗组与对照组相比视网膜血管分布规则、分支良好、新生血管密度减少,且突破视网膜内界膜的血管内皮细胞核数目明显减少(P<0.05);治疗组MMP-2染色较对照组减弱,PEDF染色较对照组增强。结论:玻璃体腔内注射2.7mL/kg Captopril能够有效抑制高氧诱导下的小鼠视网膜新生血管形成, Captopril有望成为防治血管增生性视网膜病变的一种有效的方法。
Objective: To investigate the inhibitory effect of captopril on retinal neovascularization in rats. Methods: 60 7-day-old mice were randomly divided into control group (n = 30) and treatment group (n = 30). The mice were housed for 5 days in the atmosphere of 750 ± 50 mL / L and returned to the normal air environment. The treatment group received intravitreal injection of 2.7 mL / kg Captopril once a day and the control group received 2.7 mL / kg 9 g / L sodium chloride injection continuously for 5 days. The mice in both groups were sacrificed on day 17 and their eyes were removed. ADR enzyme-labeled retinal patches, HE staining and immunohistochemistry were used to observe the changes of retinal blood vessels. The number of retinal neovascular endothelial cells was counted and the expressions of MMP-2 and PEDF protein were detected . Results: Compared with the control group, the retinal blood vessels in the treatment group were well distributed and the branches were well and the density of neovascularization was decreased. The number of vascular endothelial cells which broke the inner limiting membrane of the retina was significantly decreased (P <0.05) Weakening, PEDF staining increased compared with the control group. CONCLUSION: Intravenous injection of 2.7 mL / kg Captopril can effectively inhibit retinal neovascularization induced by hyperoxia in mice, and Captopril is expected to become an effective method for the prevention and treatment of proliferative retinopathy.