论文部分内容阅读
目的探讨尼妥珠单抗(h-R3)对体外人食管鳞癌KYSE30细胞株的放射增敏效应。方法采用培养的人食管鳞癌KYSE30细胞株行体外放射实验,分为五组:C组为空白对照;R组仅行照射;H1组于放疗前24h加用h-R3;H2组放疗前12h加用h-R3;H3组放疗时加用h-R3。MTS法检测KYSE30细胞株的生长抑制;平板克隆形成实验检测并计算平均致死剂量(D0)和准阈剂量(Dq)等相关参数;流式细胞术检测6Gy照射下各组的细胞凋亡及细胞周期分布情况。结果 h-R3对人食管鳞癌KYSE30细胞株增殖呈时间和剂量依赖性的抑制作用。h-R3作用24h后细胞增殖20%的药物浓度为98.88μg/ml。在100μg/ml h-R3作用下,H1组放射增敏比(SER)为1.43,H2组的SER为1.22,H3组的SER为0.95。H1、H2和H3组的G2/M期比例均增高、S期比例降低、KYSE30细胞凋亡率提高。结论放疗前给予h-R3能增强食管癌KYSE30细胞株的放射敏感性。
Objective To investigate the radiosensitization effect of nimotuzumab (h-R3) on human esophageal squamous cell carcinoma KYSE30 cell line. Methods The human esophageal squamous cell carcinoma KYSE30 cells were cultured in vitro and divided into five groups: blank control in group C, radiation in group R only, h-R3 in group H1 24h before radiotherapy, Plus h-R3; H3 group radiotherapy plus h-R3. MTS assay was used to detect the growth inhibition of KYSE30 cell line. The plate clone formation assay was used to detect and calculate the average lethal dose (D0) and quasi-threshold dose (Dq) and other parameters. Flow cytometry was used to detect the apoptosis and cell apoptosis Periodic distribution. Results h-R3 inhibited the proliferation of KYSE30 cells in a time-and dose-dependent manner. h-R3 24h after the cell proliferation of 20% of the drug concentration was 98.88μg / ml. Under 100μg / ml h-R3, the SER of H1 was 1.43, SER of H2 was 1.22, SER of H3 was 0.95. The proportion of G2 / M phase increased in H1, H2 and H3 groups, the proportion in S phase decreased, and the apoptosis rate in KYSE30 cells increased. Conclusion The radiosensitivity of KYSE30 cells to esophageal cancer can be enhanced by the administration of h-R3 before radiotherapy.