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OH、O_2~■和病区FA可以使猪软骨Ⅱ型胶原蛋白中羟脯氨酸、脯氨酸含量下降,而谷氨酸含量增加;且O_2~(?)和FA浓度越大,羟脯氨酸含量下降越多。OH,O_2~(?)和FA损伤的胶原蛋白的凝胶化程度和速度降低;CD谱表明其构象发生变化,α-螺旋结构含量下降;SDS-PAGE结果表明,胶原蛋白在-OH、O_2~(?)作用下发生降解生成分子量较小的肽段,而在FA作用下未见明显降解。推测FA作为自由基源在大骨节病的发生中起作用。
OH, O_2 ~ ■ and ward FA can reduce the contents of hydroxyproline and proline in pig cartilage type Ⅱ collagen, while the content of glutamic acid increased; and the concentration of O_2 ~ (FA) and FA increased The more the decrease of the content of amino acid. OH, O_2 ~ (?) And FA decreased, the conformation of the collagen changed and the content of α-helical structure decreased. The results of SDS-PAGE showed that collagen in -OH, O_2 Under the action of ~ (?), The peptides with the smaller molecular weight were degraded, but no significant degradation was observed under the action of FA. It is hypothesized that FA acts as a free radical source in Kashin-Beck disease.