2. 您的位置
3. 首页
4. 期刊论文
5. An improved Coomassie Brilliant Blue（CBB R—250） Staining To proteins in gels

An improved Coomassie Brilliant Blue（CBB R—250） Staining To proteins in gels

来源 :核技术：英文版 | 被引量 : 0次 | 上传用户：shijunfengmm

【摘 要】

：

An improved CBB staining with higher sensitivity than that of the typical CBB staining was reported.The main improvement was using a fixing step of 25% trichlor

【作 者】

：

ZHOUZhi－Dong LIUWang－Yi 等 

【机 构】

：

ShanghaiInstituteofNuclearResearch,InstituteofBiochemistryandCellBiology

【出 处】

：

核技术：英文版

【发表日期】

：

2002年4期

【关键词】

：

蛋白 CBB R-250 凝胶 Basic and low molecular weight proteins  Coomasie Brilliant Blue 

下载到本地 , 更方便阅读

下载此文 赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

An improved CBB staining with higher sensitivity than that of the typical CBB staining was reported.The main improvement was using a fixing step of 25% trichloroacetic acid(TCA) before CBB staining.For most proteins studied,the sensitivity of the improved

其他文献

Reconstruction of 12 MV bremsstrahlung spectra from measured transmission data

Transmission data for 12 MV bremsstrahlung beams produced by the process for continuous-pulsed 12 MV electrons coming from a linear accelerator hitting a 1.2mm

期刊

轫致辐射光谱线性加速器钽MONTECarlo仿真光子Electron bremsstrahlung spectra Attenuation ana

Preliminary clinical application of dopamine transporter imaging with technetium—99m TRODAT—1 and SP

The aim of the study was to demonstrate the degeneration of the dopaninergic nigrostriatal pathway in Parkinson's disease(PD) and Essential Tremor (ET) by u

期刊

多巴胺输运造影^99MTCParkinson病诊断Parkinson's disease SPECT Dopamine transporter

A small dimension intraoperative probe

This article introduces the usage of the intraoperative probe in surgical based on RGS and proposes one method to design the probe. Also, a charge-sensitive pre

期刊

小区域探针CdTe半导体探测器设计RGS Preamplifier Nuclear detector Lymph node

Coupling effect between film boiling heat transfer and evaporation drag around a hot-particle in col

Extremely rapid evaporation could occur when high-temperature particles contact withlow-temperature liquid. This kind of phenomenon is associated with the engin

期刊

耦合效应薄膜状沸腾热传导蒸汽爆炸工程安全Vapor explosion FCI (Fuel-coolant interactions) Evapo

Clinical value of dipyridamole brain perfusion imaging in the diagnosis of ischemic cerebrovascular

Using dipyridamole stress test to evaluate cerebral blood flow reserve in cerebrovascular disease (CVD). Dipyridamole stress tests were performed first, the bas

期刊

头部造影层析X射线摄影法临床诊断Cerebral ischemia Tomography Emission-computer Single-phot

Experimental study of vapor local characteristics in upward low pressure boiling tube

Radial distribution of vapor local parameters, including local void fraction, interfacial velocity, bubblesize, bubble frequency and interfacial area concentrat

期刊

沸腾管气液两相流光探针局部参数反应堆安全性Two-phase flow Optical probe Local parameter

A quantitative assessment of heart phantom motion and its effect on myocardial perfusion SPECT image

In order to study the image characteristics of motion artifacts and todetermine the relations of motion artifacts with varied motion types, and the inag-ing tim

期刊

心脏仿真运动SPECT造影Heart phantom Myocardial perfusion SPECT Motion artifacts

Moessbauer study of the effect of Nb in Fe－based nanocrystalline alloy

Microstructure of nanocrystalline alloys of Fe73.5Cu1Nb3Si13.5B9 and (Fe3Si)0.95Nb0.05 was investigated by Moessbauer spectroscopy.A Nb-rich interfacial layer w

期刊

纳米结晶合金铁铌合金Moessbauer效应Mossbauer spectroscopy Nanocrystalline alloys Residua

Positron annihilation study of effect of aluminum on iron－based amorphous alloys

Seven Fe-based amorphous alloys have been studied by Dopplerbroadening and lifetime techniques of the positron annihilation. It is shown that theparameters of p

期刊

非结晶合金铝铁合金质子湮浸Positron annihilation Amorphous alloy Defect Aluminum

Photoluminescence from neodymium silicide thin films formed by MEVVA ion source

Neodymium silicides were synthesized by Nd ion implanted into Si substrates with the aid of a metal vaporvacuum arc (MEVVA) ion source. The blender of Nd5Si4 an

期刊

MEVVA离子源离子移殖薄膜光致发光Photoluminescence Neodymium silicide Ion implantation M