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马铃薯Y病毒(PVY)是严重危害我国马铃薯生产的主要病毒之一。本研究通过RT-PCR的方法,对依据症状采集的124份马铃薯叶片样品进行了病毒检测,并对其中PVY阳性样品进行了株系类型分析。结果显示,在93份PVY阳性样品中,PVYN/NTN/N??O类型株系占60.2%,而PVYO普通株系占20.4%,还有18个样品(19.4%)显示受到两种类型PVY的混合侵染。进一步分析表明,在PVYN/NTN/N??O类型株系中,PVYNTN、PVYN??O和PVYN三种株系分别占83.8%、12.2%和4.0%;而PVYO普通株系中,PVYO-FL和PVYO-RB两种变异型各占70.3%和29.7%。本研究结果显示,PVYNTN和PVYO-FL是检测样品中主要的PVY株系,该结果为指导马铃薯PVY的防治提供一定的依据。
Potato virus Y (PVY) is one of the major viruses that seriously endanger the production of potato in our country. In this study, 124 potato leaf samples collected according to the symptoms were tested for virus by RT-PCR, and the type of the PVY positive samples was analyzed. The results showed that in 93 PVY positive samples, PVYN / NTN / N O type strains accounted for 60.2%, while PVYO common strains accounted for 20.4%, and 18 samples (19.4%) showed two types of PVY Mixed infection. Further analysis showed that the PVYNTN, PVYNO and PVYN strains accounted for 83.8%, 12.2% and 4.0% of the PVYN / NTN / N? O lines respectively, while in the PVYO common lines, the PVYO- FL and PVYO-RB two variants each accounted for 70.3% and 29.7%. The results of this study show that PVYNTN and PVYO-FL are the major PVY lines in the test samples, and the results provide some evidences for the prevention and control of potato PVY.