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目的 评价温血心脏停搏液不同间断灌注时程对心肌保护效果的影响。 方法 将４６ 只大鼠随机分为６组。组Ⅰ：温血心脏停搏液持续灌注；组Ⅱ：每２０ 分钟重复停灌５ 分钟；组Ⅲ：每２０ 分钟重复停灌１０ 分钟；组Ⅳ：停搏期一次停灌１０ 分钟；组Ⅴ：停搏期一次停灌１５ 分钟，以上每组８ 只大鼠；对照组：６ 只大鼠，心脏经Ｌａｎｇｅｎｄｏｒｆｆ 和工作心灌注各１０ 分钟后置液氮冻存。测定各组心功能，心肌肌浆网钙离子三磷酸腺苷酶（ＳＲ Ｃａ２ ＋ＡＴＰａｓｅ） 活性，心肌肌浆网４５Ｃａ２ ＋ 摄取和超微结构的改变。 结果 组Ⅱ心肌保护效果未受影响；组Ⅳ心功能恢复有影响，但心肌肌浆网泵功能保持正常；组Ⅲ和组Ⅴ心功能、心肌ＳＲ Ｃａ２ ＋ＡＴＰａｓｅ 活性和心肌肌浆网４５Ｃａ２ ＋ 摄取均有明显减退，超微结构亦受损。 结论 温血心脏停搏液间断停灌５ 分钟不影响其保护效果，一次或重复停灌１０ 分钟对心肌保护有负性作用，温血心脏停搏液停灌不宜超过１０ 分钟。
Objective To evaluate the effect of different intermittent perfusion durations of warm blood cardioplegia on myocardial protection. Methods 46 rats were randomly divided into 6 groups. Group I: continuous perfusion with warm cardioplegic solution; Group II: repeated perfusion for 5 minutes every 20 minutes; Group III: repeated perfusion for 10 minutes every 20 minutes; Group IV: : Stop the arrest of a stop for 15 minutes, more than 8 rats in each group; control group: 6 rats, heart Langendorff and working heart perfusion for 10 minutes after the set of liquid nitrogen cryopreservation. The cardiac function, SR Ca2 + -ATPase activity in myocardial sarcoplasmic reticulum, 45Ca2 + uptake in cardiac sarcoplasmic reticulum and ultrastructural changes were determined. Results The myocardial protection effect of group Ⅱ was not affected; the recovery of cardiac function of group Ⅳ was affected, but the function of myocardial sarcoplasmic reticulum pump remained normal; the cardiac function of group Ⅲ and groupⅤ, the activity of SR Ca2 +ATPase and the activity of myocardial sarcoplasmic reticulum 45Ca2 + Significantly decreased uptake, ultrastructural damage also. Conclusion Warm blood cardioplegia intermittent stop irrigation for 5 minutes does not affect its protective effect, once or repeated stop irrigation for 10 minutes has a negative effect on myocardial protection, warm blood cardioplegia suspension should not exceed 10 minutes.