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目的探讨促血管生成素-2(Ang-2)和血管内皮生长因子(VEGF)在糖尿病肾脏血管新生中的作用及其意义。方法2004年3月至2005年3月在四川大学华西医院用SD大鼠建立链唑霉素(STZ)诱导的糖尿病肾病模型,定量分析肾脏VEGF和Ang-2的表达变化,用逆转录聚合酶链式反应技术(RT-PCR)检测肾脏VEGF、Ang-2的mRNA的表达。结果糖尿病组肾脏VEGFmRNA表达从2周开始持续上调,16～20周达高峰;免疫组化显示糖尿病组各时点肾小管的VEGF着染均强于对照组;糖尿病组仅于16周和20周时探及肾组织Ang-2mRNA表达,12～24周免疫组化显示皮质区管周Ang-2着染管周微血管,整个实验期间对照组未探及Ang-2mRNA和蛋白表达;VEGF与Ang-2的改变呈正相关。结论糖尿病肾脏中后期皮质区存在Ang-2着染的新生管周微血管,VEGF与Ang-2参与糖尿病肾脏新生血管生成。
Objective To investigate the role and significance of angiopoietin-2 (Ang-2) and vascular endothelial growth factor (VEGF) in diabetic neovascularization. Methods From March 2004 to March 2005, STZ-induced diabetic nephropathy model was established in SD rats in West China Hospital of Sichuan University. Quantitative analysis of the expression of VEGF and Ang-2 in renal tissues was performed with reverse transcription polymerase The mRNA expression of VEGF and Ang-2 in kidneys was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of VEGF mRNA in the kidney of diabetic group increased continuously from 2 weeks and peaked at 16 to 20 weeks. The expression of VEGF in renal tubules in diabetic group was stronger than that in control group at all time points. The diabetic group only at 16 weeks and 20 weeks The expression of Ang-2 mRNA in renal tissues was detected by RT-PCR. Angio-2 mRNA and protein expression were detected by immunohistochemistry in 12-24 weeks in the cortex. Angio-2 mRNA and protein were not detected in the control group during the whole experiment. VEGF and Ang- 2 changes were positively correlated. Conclusions Angio-2-stained neovascular vessels exist in the mid-late cortex of diabetic nephropathy, and VEGF and Ang-2 are involved in neovascularization of diabetic nephropathy.