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目的研究在蛋白质及酶水平上的当归抽薹发育机制,为进一步阐明当归抽薹过程所发生的生理生化指标变化及预防当归抽薹提供理论依据。方法观察当归抽薹与未抽薹植株的糖代谢及过氧化物酶(POD)、多酚氧化酶(PPO)等活性的变化,测定抽薹与未抽薹当归植株游离氨基酸、可溶性糖和可溶性蛋白的量。结果在抽薹前10 d,未抽薹植株的游离氨基酸量和POD活性、PPO活性分别比抽薹植株高1.3 mg/g和0.02 U/(min.g)、0.014 U/(min.g),抽薹植株的可溶性糖和可溶性蛋白量分别比未抽薹植株高2.2%、0.5%。在抽薹期,未抽薹植株的可溶性糖和可溶性蛋白量较高,而抽薹植株的游离氨基酸量和PPO和POD活性较高。结论当归抽薹过程多个生化指标发生变化,当归栽培中应防止早期抽薹的发生。
Aim To study the mechanism of the development of Angelica sinensis bolting at the level of protein and enzyme in order to provide a theoretical basis for further clarifying the changes of physiological and biochemical indexes during the bolting process of Angelicae Sinensis and preventing the bolting of Angelica sinensis. Methods The changes of glucose metabolism, activities of peroxidase (POD) and polyphenol oxidase (PPO) in the untreated and untreated groups were observed. The contents of free amino acids, soluble sugars and soluble proteins in the bolting and non-bolting plants were determined. Results The contents of free amino acids and POD activities and PPO activities of bolting plants were 1.3 mg / g and 0.02 U / (min.g), 0.014 U / (min.g) higher than that of bolting plants respectively. Bolting plants The soluble sugar and soluble protein content were 2.2% and 0.5% higher than those of the non-bolting plants respectively. At bolting stage, the amount of soluble sugar and soluble protein of unspluted plants was higher, while the amount of free amino acids and PPO and POD of bolting plants were higher. Conclusion The biochemical indexes of the process of bolting in Angelica sinensis changed, the occurrence of early bolting should be prevented in the cultivation of Angelica sinensis.