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目的探讨已公布的不同国家不同时间分离的人类柯萨奇病毒B组3型(human coxsackievirus B3,CB3)VP1区基因特征和分子流行特点。方法在GenBank中用BLASTN软件搜索不同国家/地区、不同时间发表的CB3VP1区基因序列,共得到来自8个国家的92个可用序列,用MEGA软件计算不同毒株的核苷酸和氨基酸差异率,并构建系统进化树。结果 92株病毒可分为5个基因型(Genotype 1～5),基因型1由CB3原型株Nancy和3个法国株组成,基因型2由4株美国株组成,基因型3的地理分布最广,由德国、美国、罗马尼亚、法国、日本、荷兰、瑞典分离株组成,基因型4由5株中国台湾分离株组成,基因型5由中国山东株、云南株和台湾株组成,该基因型又分为A～E 5个亚型。不同毒株的核苷酸差异率为6.90%～24.64%(氨基酸差异率1.00%～10.20%),不同基因型之间的核苷酸差异率为15.91%～24.64%(氨基酸差异率4.02%～10.20%)。结论不同基因型/亚型可以在同一个地区流行很长时间,同一时间内不同的基因型/亚型又可在不同的地区流行,具有明显的时间和地域流行特点。
OBJECTIVE: To investigate the characteristics of the gene and molecular epidemiology of VP1 gene of human coxsackievirus B3 (CB3) isolated from different countries at different times. Methods Genomic sequences of CB3VP1 gene from different countries were searched by BLASTN software in GenBank. 92 available sequences from 8 countries were obtained. The nucleotide and amino acid differences of different strains were calculated using MEGA software. And build a phylogenetic tree. Results Ninety-two viruses were divided into 5 genotypes (Genotype 1 ~ 5). Genotype 1 consisted of CB3 prototype Nancy and 3 French strains. Genotype 2 consisted of 4 strains of American strains. Genotype 3 had the highest geographic distribution The genotype 4 is composed of five Chinese Taiwan isolates and the genotype 5 is composed of China Shandong strain, Yunnan strain and Taiwan strain, and the genotype 4 is composed of China, Taiwan, Yunnan, Divided into A ~ E 5 subtypes. The nucleotide variation rates of different strains ranged from 6.90% to 24.64% (amino acid difference of 1.00% to 10.20%). The nucleotide differences among different genotypes ranged from 15.91% to 24.64% (amino acid difference 4.02% 10.20%). Conclusion Different genotypes / subtypes may prevail in the same area for a long time. Different genotypes / subtypes may be endemic in different areas at the same time with obvious temporal and geographical epidemic characteristics.