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目的 观察热毒宁注射液(reduning,RDN)对脂多糖(LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用,探讨其作用是否与血红素加氧酶-1(HO-1)有关.方法 将48只雄性ICR小鼠平均随机分为Control组、RDN组、LPS组、LPS+地塞米松(DEX)组、LPS+RDN组、LPS+RDN+ZnPP(HO-1特异性抑制剂,锌原卟啉)组,每组8只.采用气管内滴注LPS(5mg/kg)诱导ALI;造模后24 h,采集静脉血、取肺组织和支气管肺泡灌洗液(BALF),观察各组肺组织病理学改变,测量肺湿/干重比(W/D),BALF中的细胞总数和血清LDH活性、肺组织HO-1活性和HO-1蛋白表达量.结果 与LPS组相比,LPS+RDN组大鼠肺组织损伤较轻,肺W/D、BALF中细胞总数和血清LDH活性显著降低,HO-1的活性和蛋白表达量显著升高,差异均有统计学意义(P<0.05).与LPS+RDN组相比,LPS+RDN+ZnPP组大鼠肺W/D、BALF中细胞总数、血清LDH活性显著升高,HO-1的活性和蛋白表达量减低,均差异有统计学意义(P<0.05).结论 RDN对LPS诱导的ALI有保护作用,其保护作用与HO-1表达和活性增加有关.“,”Objective To investigate the effect of Reduning (RDN) injection on acute lung injury(ALI)and heme oxygenase-1(HO-1) in LPS-·induced mice. Methods 48 male ICR mice were randomly divided into six groups:control group, LPS group, RDN group, LPS+RDN group, LPS+DEX group and LPS+RDN+ZnPP group. Mice were intratracheal instillation with LPS(5mg/kg)to induce ALI.After LPS administration for 24 h,bronchoalveolar lavage fluid(BALF)and lung tissue were collected for the histopathological examinations.Lung wet/dry(W/D)ratio,total cell counts,the content of LDH,lung tissue HO-1activity and HO-1 protein expression were examined.Results Compared with LPS group,lung W/D ratio,total cell counts and content of LDH were significantly decreased(P<0.05)while lung tissueHO-1 activity and HO-1 protein expression were significantly increased (P<0.05) in LPS+RDN group. The pathological changes of the lung were also significantly attenuated in LPS+RDN group and LPS+DEX group as compared to LPS group. ZnPP abolished the protective effects of RDN on LPS-induced ALI. Conclusion RDN has protective effect on LPS-induced ALI, and its protective effect is related to the increase of HO-1 expression and activity.