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目的:通过测定慢性乙型肝炎患者外周血淋巴细胞CD8+CD38+的表达及门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、凝血酶原时间(PT),旨在为治疗慢性乙型肝炎提供有用的参考指标。方法:流式细胞术检测38例慢性乙型肝炎、14例肝硬化患者外周血淋巴细胞CD8+CD38+细胞的百分率;同时测定AST、TBIL和PT。结果:(1)慢性乙型肝炎组CD8+CD38+、CD38+细胞均明显高于正常组(P<0.01,P<0.01),肝硬化组CD8+CD38+、CD38+细胞均明显高于正常组(P<0.05,P<0.05)。肝硬化组CD8+CD38+、CD8+细胞均明显低于慢性乙型肝炎组(P<0.05,P<0.05)。(2)慢性乙型肝炎轻、中、重各组之间比较,中度组CD8+CD38+高于轻度组(P<0.05),重度组CD8+CD38+、CD8+、CD38+均高于轻度组(P<0.05,P<0.05,P<0.05);重度组CD38+高于中度组(P<0.05);肝硬化组CD8+CD38+均明显低于轻、中、重各组(P<0.05,P<0.01,P<0.01),肝硬化组CD8+低于重度组(P<0.01)。(3)慢性乙型肝炎患者AST、TBIL、PT不正常组CD8+CD38+均高于AST、TBIL、PT正常组。结论:慢性乙型肝炎患者CD8+CD38+细胞明显升高,表明慢性乙型肝炎患者细胞免疫处于异常激活状态,并与肝功能损伤有一定的相关性。慢性乙型肝炎患者外周血淋巴细胞CD8+CD38+的测定,对病情分析和诊断有一定的临床参考价值。
Objective: To determine the expression of CD8 + CD38 + and the activity of aspartate aminotransferase (AST), total bilirubin (TBIL) and prothrombin time (PT) in peripheral blood lymphocytes of patients with chronic hepatitis B Chronic hepatitis B provides a useful reference. Methods: Flow cytometry was used to detect the percentage of CD8 + CD38 + cells in 38 patients with chronic hepatitis B and 14 patients with cirrhosis. AST, TBIL and PT were measured simultaneously. Results: (1) CD8 + CD38 + and CD38 + cells in chronic hepatitis B group were significantly higher than those in normal group (P <0.01, P <0.01). CD8 + CD38 + and CD38 + cells in cirrhosis group were significantly higher than those in normal group (P < 0.05, P <0.05). CD8 + CD38 +, CD8 + cells in cirrhosis group were significantly lower than those in chronic hepatitis B group (P <0.05, P <0.05). (2) The levels of CD8 + CD38 + in moderate, moderate and severe group were higher than those in mild group (P <0.05), and the levels of CD8 + CD38 +, CD8 + and CD38 + in severe group were higher than those in mild group (P <0.05, P <0.05, P <0.05); CD38 + CD38 + in cirrhosis group were significantly lower than those in mild, moderate and severe groups (P <0.05, P < P <0.01, P <0.01). CD8 + in cirrhosis group was lower than that in severe group (P <0.01). (3) The levels of CD8 + CD38 + in patients with chronic hepatitis B were significantly higher than those in normal group, AST, TBIL and PT normal group. Conclusion: CD8 + CD38 + cells in patients with chronic hepatitis B are significantly increased, indicating that cellular immunity in patients with chronic hepatitis B is abnormally activated and has some correlation with liver injury. Chronic hepatitis B patients with peripheral blood lymphocyte CD8 + CD38 measurement, the disease analysis and diagnosis of certain clinical reference value.