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以25份鹰嘴豆品种(系)为试验材料,通过叶面喷雾的方式进行Ascochyta rabiei菌悬液室内外人工接种,评价不同鹰嘴豆种质资源的抗病性;同时利用RAPD方法进行基因型鉴定,采用NTSYSpc 2.10t软件对分子标记结果进行遗传相似性的统计分析,并建立各品系间的亲缘关系聚类图,探讨不同鹰嘴豆品系对A.rabiei抗性与遗传多态性间的关系。室内和田间鹰嘴豆抗A.rabiei鉴定结果表明:在25份鹰嘴豆供试种质中,系选03和216均表现出稳定抗性;北园春表现出稳定中抗。通过RAPD多态性引物对25份供试种质进行PCR扩增,共获得129个扩增条带,其中多态性条带67条,多态性比例达51.94%,遗传相似系数为0.3731~0.9254。结合抗病性和遗传多态性,经方差分析表明,本研究的鹰嘴豆品种(系)对A.rabiei的抗性强弱与其遗传相似性之间无显著相关性。
Twenty-five parts of chickpea varieties (lines) were used as materials to evaluate the disease resistance of Ascochyta rabiei suspension in vitro and in vivo by foliar spraying. The resistance of chickpea germplasm resources was evaluated by RAPD method Type identification, using NTSYSpc 2.10t software molecular marker results of genetic similarity of statistical analysis, and to establish a phylogenetic relationship between the various clusters of clusters to explore the different chickpea strains between A.rabiei resistance and genetic polymorphisms Relationship. Indoor and field chickpea anti-A.rabiei identification results showed that: in 25 parts of the chickpea tested germplasm, lines 03 and 216 showed stable resistance; North Park spring showed stable resistance. A total of 129 amplified bands were obtained by PCR amplification of 25 accessions with RAPD primers. Among them, 67 were polymorphic bands with a polymorphism ratio of 51.94% and a genetic similarity coefficient of 0.3731 ~ 0.9254. According to the analysis of disease resistance and genetic polymorphism, the analysis of variance showed that there was no significant correlation between the resistance of A.rabiei and the genetic similarity of the chickpea variety (line).